2130 Journal of Applied Microscopy 



The Bacterial Flora of Freshly Drawn Milk. 



III. 



The methods employed in this work were essentially those used by Ford ; 

 a large piece of the tissue to be examined was excised with a sterile knife, 

 placed in a sterile jar, and immediately taken from the slaughter house to the 

 laboratory. Small pieces of tissue were then cut from the inside of the large 

 piece with sterilized knives, and then held in the flame of a Bunsen burner with 

 sterilized forceps until the whole of the outside of the piece was well scorched. 

 The piece was then transferred to beef bouillon or peptone whey bouillon, and 

 the preparations placed in the incubator at 37°. On the fourth day gelatine 

 plates were made from the different pieces of tissue, and the bacteria, if any, 

 were isolated. 



1. An aged cow, dried up five weeks before slaughtering, udder small, 

 with considerable fatty tissue. All organs perfectly healthy and normal. 



r ■ Bouillon Peptone Whey 



Liver,— I 



Subsequent plating and sub-cultures gave 

 B. mesentericus vulgatus. 

 B. snbtilis, and a Micrococcus, identity not established. 



J.J.,, Bouillon Whey Peptone 



Plates gave colonies of 

 B. subtilis. 

 Micrococcus (sp ?). 



2. An aged cow, dry for some time, the butcher not knowing the exact 

 length of time. Udder of a fair size and well formed. All organs apparently 

 healthy and normal. 



r . Bouillon Whey Peptone 



Liver, — I ■' . 



Plates gave colonies of 

 B. lactis icrogenes. 

 Proteus. 



Udder, — 



Bouillon Whey Peptone 



3. An aged cow. dry for four weeks previous to slaughter. Udder fair 

 size. Organs normal and apparently healthy. 



^ . Bouillon Whey Peptone 



Gelatine plates gave colonies of 

 B. subtilis. 

 A spore bearing bacillus, which produces no effect in milk. 



Bouillon Whey Peptone 



Udder, — , -^ , 



Gelatine plates gave cultures of 

 Micrococcus varians lactis. 



