2132 



Journal of Applied Microscopy 



Elementary Medical Micro-Technique for Physicians and 

 Others Interested in the Microscope. 



Copyrighted. 



XII. 



BLOOD EXAMINATIONS FOR THE MALARIAL PARASITE. 



Clean a finger or lobe of the ear thoroughly with soap and water, alcohol 

 and ether. Dry and puncture with the blood lancet. Collect a small drop of 

 blood on a clean cover and immediately mount it on a clean slip, blood side 

 down, as a fresh preparation. The drop of blood should be rather small and 

 mounted quickly so that a thin layer of cells may be observed. The malarial 

 organisms if present may be seen. 



Fig. XXI. 



Fig. XXII. 



Figs. XXI, XXII. — Plasmodium Malariae. Stain special hematoxylin. Magnified 1800 diameters. Bausch & 

 Lomb iB-in. oil immersion objective ; Bausch & Lomb compensating ocular No. 2. 



Jenner's stain gives good results. Ap- 

 ply as directed in staining blood. The 

 malarial parasites will stain blue. 



Thionin is a reliable stain for the or- 

 ganisms of malaria. It is used as follows : 

 Spread a thin film of blood on a clean 

 cover glass. Immerse it for one minute in 

 10 c. c. of 95 per cent, alcohol to which 1 

 drop of formalin is added at the time of 

 using. Rinse the cover in water, dry be- 

 tween filter papers and stain for fifteen 

 seconds in 



50 per cent, alcohol saturated 



with thionin - - 20 c. c. 



2 per cent, solution of carbolic 



acid in distilled water 100 c. c. 



Fig. XXIII.— i;l(.n,l l.ynipliatic Leukemia. Ehr- 

 lich's triacid stain, showin.e large mononuclear, 

 polynuclear and neutrophilic corpuscles. Also 

 red corpuscles, the relation of wliite corpuscles 

 to the red being about one to eight, i^-in. oil 

 immersion objective ; Zeiss projection ocular 

 No. 4. 



