and Laboratory Methods. 



•2181 



The accompanying engraving is from a photograph of a live anesthetized 

 Daplmia of medium size, as it appeared when projected on a screen and a class 

 was studying the pulsations of its heart. Only partial anesthesia had been 

 induced by placing the animal in a mixture of six parts water to one of one per 

 cent, chloretone solution, in order that slow normal movements of the append- 

 ages might be studied. A slight movement of the antennae occurred during the 

 twenty-five seconds exposure of the photographic plate. Sunlight and a three- 

 quarter inch objective (Leitz No. 3) were used in the manner described in May 

 and June (1902) issues of this Journal in projecting the image of the live 

 Daphnia on the screen. The photograph was made with a Unar lens (B. & L. 

 Opt. Co.), on Cramer's isochromatic instantaneous plate. 



Cyclops and Gammanis. — These and many other species are to be treated in 

 essentially the same manner as Daphnia, the only difference being in the propor- 

 tion of chloretone to water, and this is readily determined by experiment. 

 University of Chicago. A. H. CoLE. 



The Bacterial Flora of Freshly Drawn Milk. 



IV. 



BIBLIOGRAPHY. 



' Scbultz, L. Ueber den Scmutzgehalt der 

 Wiirzbuger Markt-milch und die Herkunft 

 der Milch bacterien (Archiv. f. Hygiene Bd. 

 XIV, p. -iliO, 1892). 



2 Qernhardt, E. Quaniitativ Spaltpilzun- 

 lersuchungen der Milch. (Dissert). Dorpat, 

 1893. 



■ Freudenreich, (Ed. Von). Dairy Bacte- 

 riology, translated from the German by I. 

 R. Ainsworth Davies, London, 1895. 



' Bolley, H. L. and Hall. C. M. ( Centbl. Bakt. 

 und Par. (1895), No. 22-23, pp. 788-795). 



• Russell, H. L. Dairy Bacteriology p. 42, 

 1893. 



"■ Grotenfelt, Gosta. The Principles of Mod- 

 ern Dairy Practice, translated by F. W. 

 Woll, p. 23. 



■ Rotcb, Dr. T. M. Transactions of the Asso- 

 ciation of American Physicians, 1894. 



>* Moore, V. A. Preliminary Observations on 

 the Number and Nature of Bacteria in 

 Freshly Drawn Milk. Twelfth and Thir- 

 teenth Annual Reports, Bureau of Animal 

 Industry, U. S. Department of Agriculture, 

 p. 261. 

 " Conn, W. H. Bulletin No. 25, U. S. De- 

 partment of Agriculture, OiTice of Expt. Sta., 

 p. 9. 



1" Harrison, F. C. Twenty-second Annual 

 Report, Ont. Ag. Coll., p. 107, 189(5. 



1 ' Palleske, A. Ueber den Keimgehalt der 

 Milch gesunder Wochnerinnen, Virchow's 

 Archiv. Bd. CXXX, p. 185, 1894. 



'2 Honigmann, F. Bacteriologische Unter- 

 suchungen iiber Frauenmilch. Zeitschr. f. 

 Hygiene, Bd. XIV, p. 207, 1893. 



Ontario Agricultural College. 



■ Knochensteirn, H. Ueber den Keimgehalt 

 der Dorpater Marktmilch, nebst einigen bac- 

 teriologischen Untersuchungen von Frauen- 

 milch, Dorpat, 1893 (Inaug. — Diss.). 



' Ringel, L. Ueber den Keimgehalt der 

 Frauenmilch (Miinchenermed. Wochenschr., 

 No. 27, 1893). 



■ Bulletin 158, Cornell University Agricul 

 tural Experiment Station. 



' Journal of Applied Microscopy, Vol. I, No 



12, p. 205. 

 " Bulletin 178. Cornell University Agricul 



tural Experiment Station. 

 "^ Chester, F. D. Determinative Bacteriol 



ogy. New York, 1901. 



" Wright. Memoirs of the National Acad 



emy of vSciences, VII, 1895. 

 " Ford, W. W. The Bacteriology of Healthy 



Organs. Transactions of the Association 



of American Physicians. Vol. XV, p. 389, 



1900 ; al.so in The Journal of Hygiene, Cam 



bridge, 1901. 



' Conn, H. W. Classification of Daiiy Bac- 

 teria, Storrs (Connecticut) Agr. Expt. Sta. 



Report for 1899. 

 - Fokker, H. P. Ueber kie bacterienvernich- 



tenden ergenschafter der Milch. Fotsch. d. 



Medicin 130, VIII, p. 7, 1890. 



• de Freudenreich. De Taction bactericide 



der lait. Ann. de Micrographin III, p. 116, 



1891. 

 ^ Brieger and Ehrlich. Zeits. f. Hyg. B. 



XII, 189;!. 

 5 Wassermann. Zeitz. f. Hyg. 131, 18, 235- 



F. C. Harrison. 



M. CUMMING. 



