and Laboratory Methods. 



2183 



embedding, which are facilitated by the body heat. Sectioning the specimen, 

 staining and mounting are the next steps. 



Clamp the block bearing the embedded specimen in the object holder of a 

 microtome. Set the knife obliquely so that two-thirds of its edge may be used 



Fig. XXVI. — Carcenoma of the breast, showing invasion of cells. 

 Stain, haematoxylin and eosin. Magnified 40 diameters ; 2inch 

 objective; Zeiss projection ocular No. 4. 



in cutting a section. Keep the specimen wet with the 85 per cent, alcohol and 

 the knife flooded with the same fluid. Cut the sections from ten to twenty 

 microns thick and transfer them to a watch-glass of 85 per cent, alcohol, using 

 a large camel's hair brush for the purpose. 



Fig. XXVIL— Epithelioma of the lip. Stain, haematoxylin and eosin. 

 Magnified 60 diameters; -j-inch objective; Bausch & Lomb 

 compensating photo ocular No. '2. 



If no sliding or hand microtome is at hand, satisfactory free hand sections 

 may, with a little practice, be made. Hold the specimen in the left hand and a 

 razor in the right. Begin at the heel of the razor and draw it towards you, rest- 



