and Laboratory Methods. 2195 



CURRENT ZOOLOGICAL LITERATURE. 



CHARLES A. KOFOID, University of California. 



Books and Separates of Papers on Zoological Subjects should be Sent for Review to Charles A. 

 Kofoid, University of California, Berkeley, California. 



Bolles Lee, A. Nouvelles recherches sur le The material used was the testis of 

 Nebenkern et la rtfgression du fuseau cary- tt t i -i i. ,. -i i • 



ocinetique. La Cellule 20: 181-216, i pL, ^ehx, and the results are attributed in 



i9°2- part to the use of Bouin's formol-picric 



fluid, of which the following is the formula : 



Saturated aqueous solution of picric acid, - 30 pts. 

 Formol, ------- 10 " 



Glacial acetic acid, . - . . 2 " 



Sections were fixed to the slide by the water method and then stained by 

 Benda's iron haematoxylin. After decolorizing, the sections are washed in water 

 and then stained for one and one-half minutes in 0.5 per cent, aqueous solution 

 of acid fuchsin. This usually overstains, but is easily reduced by washing in 

 slightly alkaline water. Ordinary tap water generally suffices. The result sur- 

 passes that obtained after the use of Flemming's classic fluid. Chromatin and 

 the acrosome are black, while spindle fibers, the nebenkern and the cytoplasmic 

 reticulum are red. The author finds fixation by Bouin's fluid most admirable, 

 very uniform, and commends it highly for use in conjunction with staining 

 method he employed. c. a. k. 



Monti, Rina ed Monti, Achille. Le ghiandole Animals were killed at intervals during 



gastriche delle Marmotte durante il letargo ° 



invernale e I'attivita estiva. Richerche Lab. winter lethargy and vernal activity. 

 Anat. Roma e altrl Lab. Biologici 9: 1-25 p^^^g ^f ^^le gastric glands and cana- 

 ( Reprint), pis. 8, 9. 1902. ° ** 



liculi of the delomorphous cells were 



demonstrated by the Golgi method. Small pieces of the gastric wall 

 were placed for two days in a mixture of 3 per cent, potassium bichro- 

 mate (8 parts) and 1 per cent, osmic acid (2 parts), and subsequently 

 treated with nitrate of silver. Better results were obtained with rejuvenated 

 material. This was fixed in osmic-bichromate and afterwards kept in the 

 simple bichromatic solution. Whenever desired the pieces are taken from the 

 bichromate solution and placed for 5 or 6 days in a saturated aqueous solution of 

 copper sulphate filtered and diluted one-half and renewed daily. From this they 

 are passed to an osmic-bichromate mixture (3 per cent, bichromate of potash 9 

 parts, 1 per cent, osmic acid 1 part) for 24 hours and then to 0.75 per cent, silver 

 nitrate. The impregnation of the ducts begins in 24 hours and lasts for more 

 than 10 days. Embedding in paraffin must be done rapidly. 



The Golgi-Zimmermann method was also used. Sections of Golgi material 

 on slides are brought to alcohol and then to a mixture of 1 part 0.75 per cent. 

 NaCl in water and '2 parts 96 per cent, alcohol for 10 minutes. They are then 

 returned to alcohol in diffuse light for a day and subsequently stained with 

 thionin with safranin for contrast. 



