2196 Journal of Applied Microscopy 



Sections of material fixed in osmic-bichromate were also stained in saturated 

 aqueous solution of rubin, washed in water, placed for a few seconds in a mixture 

 of equal parts of alcohol and saturated aqueous solution picric acid, washed again 

 in water and then stained for not more than half a minute in 0.5 per cent, aqeuous 

 solution of methyl-green. By this method the nuclei are stained green, the gran- 

 ula, especially in the delomorphous cells, red, and the protoplasm a greenish 

 gray. An inverse coloration was produced by Galeotti's method. Sections of 

 material fixed in Hermann's fluid were stained in saturated solution of acid fuch- 

 sin in anilin water at 60° C, then washed in water, decolorized for a few seconds 

 in picric alcohol, washed again in water and counterstained in a 0.5 solution 

 of methyl-green in 50 per cent, alcohol. 



For differentiation of the delomorphous and adelomorphous cells, the author 

 employed Heidenhain's sublimate (alcohol 60 c. c, chloroform 30 c. c, acetic acid 

 10 c. c, sublimate Tgms.) and stained sections in Mayer's hgemalum and Congo 

 red, or Biondi's triacid, or Heidenhain's iron haematoxylin followed by acid 

 fuchsin. c. a. k. 



Hjort, J., and Dahl, K. Fishing Experiments The Otter-seine devised by Dr. Peter- 

 in Norwegian Fiords. Rep. Norweg. Fish sp^ /^ee this Journal Vol IV No m 

 and Marine Invest., 215 pp., 32 figs., 3 maps. sen (^see inis journal \ Ol. i V , i\o. li; 



Kristiania, 1900. has been redescribed by Dr. Hjort 

 Petersen, C. G. J. Alterations and Improve- -^^ considerable detail, and he com- 

 ments on Otter-semes for Zoological Fur- 

 poses. Rep. Danish Biol. Sta. II : 41-45, mends its utility for zoological coUect- 



'902. ing. The difficulty caused by the net 



cutting into soft bottom was overcome by raising the ground rope a few inches 

 from the bottom by means of glass floats attached to cords about six inches in 

 length. These cords were fastened to the ground rope and also to light 

 sinkers. The sinkers follow irregularities in the bottom, and the floats keep the 

 ground rope free from the ooze. Small seines constructed on the pattern of the 

 otter trawl, with silk gauze or bobinet for mesh, were used in shallow water and 

 for surface fishing. For deep water a small bag with fine mesh was placed 

 outside and behind the bag of the main seine, and served to retain the smaller 

 organisms without carrying the strain of the heavy load, or in any way decreas- 

 ing the effectiveness of the coarser seine. 



Dr. Petersen records the use of his otter-seine in many seas with uniform 

 success, but suggests that, owing to complications in hanging the seine, the 

 pattern seine be purchased from Danish makers, and offers his services to 

 intending purchasers. The cost of a complete net is about $25.00. He has 

 found that the trouble caused by the seine cutting into soft bottom may be 

 obviated by cutting down the length of the arms to mere stumps and widening 

 the mouth of the seine by stretching the meshes upon the elongated head and 

 foot ropes. The twisting of the crow-foot, caused by turns in the tow-rope, was 

 prevented by using shackles with ball-bearings. Advice is also given for 

 maneuvering the vessel in lowering and raising the seine, and suggestions are 

 made for keeping the seine upon the bottom. c. a. k. 



