and Laboratory Methods. 2109 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



JOSEPH H. PRATT, Harvard University Medical School. 



Books for Review and Separates of Papers on these Subjects should be Sent to Joseph H. Pratt, 

 Harvard University Medical School, Boston, Mass. 



Brinckerhoff and Tyzzer. On the Leucocytes There are five varieties of leucocytes 



of the Circulating Blood of the Rabbit. in the peripheral blood of the rabbit. 



Journal of Medical Research, 7: 17^-180, -. ^ , rr-,, r 



JQ02. -J y j_ Lymphocytes. These form 



45 to 55 per cent, of the total number 

 of leucocytes. Size, 7 to 9 //. Nucleus, round; chromatin granules in masses, 

 having a mural arrangement. Protoplasm, non-granular and strongly basophilic. 

 They originate in the germ centers of the lymph-nodes. 



II. Large mononuclars. Two to eight per cent, of total number. Size, 12 

 to 16 //. Nucleus, oval or curved, vesicular. Protoplasm, non-granular, slightly 

 basophilic. The writers think these cells arise from the endothelium of the 

 lymph and blood vessels. 



III. Amphophiles. These are the homologues of the neutrophilic leucocytes 

 of human blood. Ehrlich defines them as cells possessing granules which take 

 either the acid or basic dye. The writers, however, were not able to stain the 

 granules with the basic stain. Forty to fifty per cent, of the leucocytes belong to 

 this class. Size, 10 to 12 /i. Nucleus, polymorphous ; chromatin, in masses, 

 murally arranged. Protoplasm, granular; granules small, ovoid, oxyphilic. 

 They arise from the amphophilic cells of the bone-marrow. 



IV. Eosinophiles. One-half to one percent. Size, 12 to 14 //. Nucleus, 

 polymorphous ; chromatin, in masses, murally arranged. Protoplasm contains 

 large ovoid, oxyphilic granules. They are formed from the eosinophilic cells of 

 the bone-marrow. 



V. Mast-cells. Four to eight per cent. Size, 10 to 12 jj. Nucleus, poly- 

 morphous, poor in chromatin. Protoplasm contains small, spherical, basophilic 

 metachromatic granules. 



In making dried blood films, a small drop of fresh blood was touched with 

 the edge of a clean cover-slip. The edge of this cover, with the drop of blood 

 adhering to it, was pressed against the surface of a second cover-slip, the two 

 making an angle of about forty-five degrees. Capillary action spread the drop 

 of blood along the angle formed by the two glass surfaces, and the blood was 

 then evenly distributed on the second cover by sweeping the first across its sur- 

 face. This procedure yielded more satisfactory results than the common 

 method of Ehrlich. j. h. p. 



Nicholls. Simple Adenoma of the Pancreas Primary tumors of the pancreas have 

 Arising froni an Island of Langerhans. The only occasionally been noted. Segie, 

 J. of Med. Research, 8 : 385-395, 1902. ■' ■' ° 



in eleven thousand five hundred au- 

 topsies at Milan, found only one hundred and thirty-two instances, a proportion 

 of one hundred and fourteen-hundredths per cent. In these cases carcinoma 

 was observed one hundred and twenty seven times; sarcoma, twice ; cysts, 



