and Laboratory Methods. 2251 



Jochmann. Ueber neuere Niihrboden zur This author has found that a medium 

 Zuchtung des Tuberculoseerreger's, etc. ^jj-j^ ^^^ 3^,^^ reaction has a favorable 

 Hyg. Rund., p. 969, 1900. 



effect upon the growth of the tubercle 

 bacilli. The most vigorous development is obtained from the blood serum of 

 sheep, cattle, and man, to which lactic acid had been added in the proportion 

 of ten drops of a 1 per cent, solution to 50 c. c. of the serum. The above 

 serum should give a neutral reaction with the litmus before the lactic acid is 

 added. h. w. c. 



Bang. Ueber die Abtodtung der Tuberkel- Bang has contributed a series of care- 

 bacillen bei Warme. Zeit. f. Thiermed. 7: £^| experiments to the question as to 

 81, 1902. 



the temperature at which the tubercle 



bacilli in milk are rendered innocuous. The claim made by Smith, some years 

 ago, that tuberculous milk, heated for a time to 60° C, in such a way to avoid 

 the formation of the scum, is made incapable of producing tuberculosis, 

 has been strongly contested. The very high rank of Prof. Bang makes it 

 especially interesting to find that he confirms Smith's observation practically /;/ 

 fofo. He finds that milk in closed tubes, heated to 60° for five minutes, if 

 inoculated, produces tuberculosis, but to a moderate extent; if heated for fifteen 

 minutes, the effect is very much reduced. Milk heated to 65° for five minutes 

 is rendered entirely innocuous, and the same result follows heating at 75°, 80° 

 and 85°. He found, however, that milk heated for two minutes at 60° gave 

 only negative results when this milk was used in feeding experiments, thus con- 

 firming the conclusion reached by Smith, that a temperature of 60° for a com- 

 paratively few minutes is quite sufiiicient to render milk incapable of producing 

 tuberculosis when it is subsequently used as food. 



A series of experiments on the same subject has been described by Hesse in 

 Zeit. f. Thiermed., p. o'll, 1902, and the results were practically identical, show- 

 ing that a heating to 60° for twenty minutes renders the milk harmless, not only 

 so far as concerns tuberculosis, but also in destroying the infectious agency of 

 cholera, typhoid and diphtheria. h. w. c. 



Dorset. The Use of Eggs as a Medium for The method adopted by the author is 

 the Cultivation of the tubercle bacilli. ^^ ^-^^ together the white and the yolk 

 American Med. 3: 555,1892. '^ 



of eggs in test tubes and then, by keep- 

 ing the material in the tubes at 70° C. for four hours upon two successive days^ 

 it is both sterilized and coagulated. The use of the yolk alone does not pro- 

 duce a very abundant growth of the tubercle bacilli and the use of the white 

 alone is quite unsatisfactory. A couple of drops of sterilized water is placed in 

 each of the tubes to moisten the material, and then the tuberculous matter is in- 

 oculated upon the surface of the medium. An abundant and satisfactory growth 

 has been obtained from tuberculous material from guinea pigs. h. w. c. 



