2294 Journal of Applied Microscopy 



f 1 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



JOSEPH H. PRATT, Harvard University Medical School. 



Books for Review and Separates of Papers on these Subjects should be Sent to Joseph H. Pratt, 

 Harvard University Medical School, Boston, Mass. 



Scott, G. Formalin or Other Fixing Vapor, According to Brinckerhoff and Tyzzer 



Followed by Absolute Alcohol, as a Wet and Professor G. Sims Woodhead this 

 Method for Blood Films. Journal of Path- ^u j • n i. u 



ology and Bacteriology, 7 : 131-136, 1901. "^^^hod gives excellent results : 



1. Hold the wet film, wet side 

 down, in the mouth of a wide bottle, half filled with ordinary 40 per cent, solu- 

 tion of formic aldehyde, for about five seconds. 



2. Drop, still wet, film side downwards, into absolute alcohol. Leave fifteen 

 minutes, or, if more convenient, as long as forty-eight hours. 



3. Blot off excess of alcohol, and move cover-glass to a dry part of the 

 blotting paper. 



4. Immediately, before any drying occurs, drop on a few drops of eosin- 

 methylene blue stain (Jenner's stain) ; cover with a watch glass ; stain for two 

 minutes, no longer. 



5. Allow excess of stain to run ofif the cover-glass, and rinse at once in a 

 bowl of distilled water. 



6. Blot off excess of water. 



7. Dehydrate very rapidly in absolute alcohol, merely dipping in and with- 

 drawing as quickly as possible. 



8. Wash off alcohol in first xylol rapidly ; wash in second xylol ; drop on 

 fresh xylol. 



9. Mount in xylol balsam. 



Scott insists upon the importance of using only pure distilled water to wash 

 off excess of stain, and cover-slips, which are quite free from acid. The blood 

 film must not be allowed to dry at any stage. j. h. p. 



Brinckerhoff and Tyzzer. On Amphophile Leu- Ehrlich and others have held that the 

 cocytogenesis in the Rabbit. Journal of granular leucocytes are formed in the 

 Medical Research, 8: 449-495, 1902. , „, 1^ j ^ -i j 



bone marrow. The results detailed 



in this exhaustive study bring additional support to this view. The bone mar- 

 row is regarded as the chief source, if not the only source, of the amphophile 

 leucocytes of the rabbit. 



In the early stages of mild peritonitis, produced by the injection of a dilute 

 suspension of turpentine, the amphophile leucocytes accumulate in the mesen- 

 teric vessels and in the surrounding tissues. Coincident with this the number 

 in the peripheral blood decreases, but later increases, and the bone marrow be- 

 comes depleted of adult amphophile leucocytes. 



The following method was employed in order to obtain satisfactory prepara- 

 tions of mesentery and omentum. The fresh mesentery was spread out over 

 the end of a segment of wide calibre glass tubing, or better, the severed neck of 



