and Laboratory Methods. 2295 



a wide mouthed bottle, and tied on by strong thread. The adjacent mesentery 

 and attached intestine was then cut away and the bottle top, with its " drum 

 head " of mesentery, dropped into the fixing fluid. The mesentery remained on 

 the bottle top through all the steps of fixation, hardening and staining until the 

 final clearing with xylol previous to mounting. A sheet of cigarette paper was 

 then spread over the exposed surface of the mesentery and with a fine pointed 

 scissors a square or circle of mesentery and of paper was cut out. The two 

 were then laid on a slide with the paper uppermost. One application of a blot- 

 ter would remove enough xylol from the paper to permit of its being seized with 

 a fine pointed forceps and stripped off the mesentery, leaving the latter spread 

 evenly on the slide. The mesentery was then treated with balsam and covered 

 with a cover glass. By this method all distortion and wrinkling of the mesentery 

 was avoided. 



The adult amphophiles are formed from the undifferentiated marrow cells, 

 which cannot be distinguished from lymphoid cells. They are of small size 

 with a round vesicular nucleus and a moderate amount of protoplasm which 

 contains a basophilic cytorecticulum. Every intermediate form is found in the 

 marrow between these simple cells and the adult amphophile. In addition to 

 the formation of amphophiles by differentiation of these simple cells they may 

 arise by the multiplication of the myelocytes. The amphophiles do not 

 multiply. 



The supply of amphophile leucocytes in the circulation is kept up by the 

 entrance of fully formed cells from the bone marrow. The hypothesis is ad- 

 vanced that the chemical composition of the blood serum exerts a chemiotactic 

 influence on the leucocytes in the bone marrow and draws them into the circula- 

 tion. A leucocytosis is only an indication of an increase of the chemiotactic 

 substances in the blood. j. h. p. 



, TT , J- T^ ■ , , Ti, It has been shown that in icterus 



Lang. Ueber die Resistenz der rothen Blut- 



korperchen gegen hypoisotonise NaCl Los- and infectious diseases there is an in- 

 ungen bei Magenkrebs. Zeitsch. fur klin. creased resistance of red blood corpus- 

 Med. 47 : 153, 1902. ^ 



cles to hypotonic sodium chloride solu- 

 tions. Lang has devised a method of determining the resistance of red blood 

 corpuscles against the hypotonic sodium chloride solution. The method depends 

 upon the fact that as red blood corpuscles go into solution, the mixture becomes 

 transparent. He studied seventeen cases of carcinoma of the stomach and 

 twenty other cases of stomach disease, and found that resistance of erythrocytes 

 to lowering of osmic pressure of the surrounding fluid is generally greater in 

 cases of carcinoma than in other stomach diseases. He attributes this change 

 to toxic products formed by the neoplasm. This condition becomes more 

 marked as the disease progresses. The author suggests the hypothesis that a 

 toxin formed by the new growth causes at first hemolysis, but later the red blood 

 corpuscles grow more resistant to the toxin, and this same resistance acts against 

 hypotonic solutions. j. h. p. 



