2342 Journal of Applied Microscopy 



employed, the author added a little acetic acid to 70 per cent, alcohol, which 

 will then dissolve sufficient orange G to stain the sections deeply in one minute. 



c. A. K. 



^ ^ , ^ ^ ^ . . This is a study of the process of infec- 



Leger, L., et Duboscq, 0. Les Gregarines et • i r i 



I'epithelium intestinal chez les Tracheates. tion by the parasite and of the means 

 Arch, de Parasitolgie, 6: 377-473- pls- 2-6, ^f defense by the host. Ripened sporo- 

 1902. 



cysts from parasitized individuals were 



fed to uninfested ones and the process of attachment of the sporozoites to the 

 intestinal epithelium and the growth of the parasite traced to the adult condition. 

 In some cases, as in Sfylor/iync/ii/s, the sporocysts are not mature when voided. 

 When freed from impurities they are placed in a sterilized moist chamber on a 

 fragment of charcoal, where they reach maturity without alteration. Thymol 

 used by Labbe as an antiseptic with the Coccididia is not recommended by the 

 authors for the Gregarinida. Ripe sporocysts placed in the gastric juice of their 

 normal hosts will dehisce in a short time as a rule. With Diplocystis, however, 

 dehiscence was secured only within the digestive tract of the animal. In this 

 case it was found possible to substitute for the normal host a closely related 

 species, Grylhis campestris. Artificial infection is best accomplished by keeping 

 the animals to be infected without food or water or even moisture for some days. 

 The ripe sporocysts are then mixed with a small quantity of food and fed to the 

 famished animal. At desired intervals the infested insects are killed, their mid- 

 guts dissected out and spread out in the killing fluid. Flemming's fluid, saline 

 or acetic sublimate, and von Rath's fluid were used for fixing and hsemalum with 

 various counter stains, iron haematoxylin, safranin with picric acid, light-green, 

 or picroindigo carmine, and Flemming's triple stain for coloration. c. a. k. 



Maier, H. N. Ueber den feineren Bau der The infusoria were killed with osmic 

 Wimperapparate der Infusorien Arch, fur ^^j^j f^j^gg ^^d then treated with a 5 

 Protistenkunde, 2 : -73-379, Taf. 3, 4, 1903. • r 1 r l 



per cent, solution of soda, after the 



method of Schewiakoff, for study of the arrangement of the cilia. The relation 

 of the cilia to the cytoplasm was studied in sections. The larger infusoria visi- 

 ble to the unaided eye were picked up individually with a pipette and transferred 

 to the killing fluid. The smaller species were secured by mixing equal quanti- 

 ties of the water, or infusion containing them, and of the killing fluid. The 

 mixture was then sedimented in a centrifuge. Cold sublimate-alcohol (1 part 

 absolute alcohol to '1 parts of a 5 per cent, solution of sublimate in 0.6 per cent, 

 salt solution) gave the best service as a fixing agent, though strong Flemming's 

 mixture was excellent for plasmatic structures. After fixing the animals were 

 transferred with a pipette to a glass tube 10 to 15 mm. long and about "2 to 3 mm. in 

 diameter, the lower end of which had been closed by a plug of fern-wool. The 

 tube is then filled with the desired fluid and the upper end is also closed in a 

 similar fashion. The tube and its contents are carried up through the alcohols 

 to chloroform, chloroform-paraffin, and finally to pure melted paraffin. The 

 upper plug is then removed to hasten the evaporation of the chloroform. After 

 standing for some time in the paraffin the tube is removed and the paraffin 

 allowed to cool. When cold the tube is slightly warmed in the hand and the 



