2440 Journal of Applied Microscopy 



When any of the first three of these solutions are used they are to be injected 

 into the blood vessels through some large and accessible artery, usually the 

 femoral. Keiller recommends that pressure injection be used, rather than the 

 ordinary brass syringe and hand pressure, and that the pressure be maintained 

 for a long time. In this way large quantities of the fluid may be injected. The 

 success of the preservation depends in a large measure in getting in a large 

 quantity of the fluid. The specimens, after injection, may be kept indefinitely 

 on the tables without immersion. They should be kept covered with cloths, to 

 prevent drying out. The reviewer has used solutions '1 and 4 with very satis- 

 factory results in preserving cats for dissection. No mould forms on the speci- 

 mens, as happens when formalin is used without carbolic acid for injection. 



For a colored injection mass Keiller recommends the following : 



Fortniila 5. — Colored injection mass : 



A. Potassium bichromate ----- 3 ounces 

 Water ----..-. i pint 



B. Lead acetate (commercial) - - - - 6| ounces 

 Water ..--..-. 1 pint 



C. Gelatine (commercial) ----- 4i ounces 

 Water -.----.. i pint 



Dissolve A, B, and C in separate stone jars immersed in a large fish kettle 

 and raised very nearly to the boiling point. Strain the gelatine solution through 

 a fine wire strainer into a vessel capable of holding two quarts ; add to this, 

 while hot, the hot bichromate solution (also strained), stir well and add gradu- 

 ally, while hot, the acetate of lead solution (also straining it). Inject, while hot, 

 with a brass anatomic syringe, using about as much force as you can exercise 

 with the hand. The fluid should be so hot that you require to protect with a 

 cloth the hand holding the syringe. A human body requires about a pint of 

 this mass. The specimens need not be warm and will be ready for dissection 

 after twenty-four hours. Bodies should be injected with the color mass not less 

 than a week after the preservative fluid has been injected. R. p. 



Michaelis^ describes a method of fas- 



A New Method of Fixing Paraffin Sections ^ • ^. ^ ^u i j \^- \^ 



, „,. . " tening sections to the slide which com- 



to the Slide. , . , , ,, 



bines the advantages of the dry albu- 

 men and the water methods. The sections are floated on the surface of warm 

 water (ca. 45° C.) until they have straightened, and are then lifted on a clean glass 

 slide. Drops of adherent water are removed with filter paper. Then a piece of 

 smooth writing paper is pressed firmly on the sections as they lie on the slide. 

 The sections, of course, stick to the paper. The paper should then be trimmed 

 down with scissors until none projects beyond the edges of the paraffin of the sec- 

 tions. A slide is then coated with albumen fixative in the ordinary way, and the 

 sections (attached to the paper) pressed firmly down on this coated slide. The 

 albumen is then coagulated by heat and the paraffin melted over a flame in the 

 ordinary manner. When the slide is dipped into xylol the paper falls to the 

 bottom of the tube as the paraflin is dissolved. 



The advantages of the method are that the sections are perfectly flat and 

 firmly fastened to the slide. It is especially useful in handling large sections. 



R. p. 



' Centralbl. f. Allgem. Path. 14: 264-265, 1903. 



