and Laboratory Methods. 2583 



CURRENT ZOOLOGICAL LITERATURE. 



CHARLES A. KOFOID, University of California. 



Books and Separates of Papers on Zoological Subjects should be Sent for Review to Charles A. 

 Kofoid, University of California, Berkeley, California. 



Rubaschkin, W. Zur Morphologic des Gehirns '^^'^e brains of Kaua, Biifo, Salamandnt 

 der Amphibien. Arch. f. mik. Anat. u. Ent- and 7>7/tf// were Studied by impregna- 

 wick. 62; 207-244, Taf. 12, n., loo^. . . , .. ^ . 



tion processes with some modification 



of wellknown methods. The blood system of the animals used was injected 

 either intra vitam or post mortem with a 5 per cent, solution of potassium 

 bichromate, or with Lavdowsky's saturated solution of neutral chromate of potash 

 or rubidium. Ten or fifteen minutes after injection the nervous system was 

 removed, cut into pieces .5 to 1 cm. in length and placed for \'l to is hours in 

 the following mixture, made just before using. 



5 per cent, bichromate of potash - - 100 

 1 per cent, osmic acid - - - - 15 



40 per cent, formaldehyde (Schering) - 5 



This mixture darkens in 24 hours after mixing and loses its efficacy as a fixing 

 agent. Clearness of the preparation is also enhanced if acetic acid to a strength 

 of 1 per cent, is added to the mixture. The superficial layers of the tissue will 

 then be found free from precipitates. If, however, the acetic acid is added 

 to the injecting fluid also the tissues throughout will be freed from precipitates. 

 Impregnation in a strong solution ("2 per cent.) of silver nitrate for 8 to 12 hours 

 was followed by the usual embedding in celloidin. For clearing purposes tur- 

 pentine concentrated by evaporation to a syrupy consistency was used so as to 

 avoid the shrinking which follows the use of xylol or toluol. c. a. k. 



Neidert, L. und Lieber, A. Ueber Bauund Ent- The size of the animal is here no crite- 

 wicklung der weiblichen Geschlechtsorgane . , , 1 i 1 



de Amphioxus lanceolatus. Zool. Jahrb. ^lon to the Stage of sexual develop- 

 Abth.f. Anat. u. Ont. 18: 187-227, Taf. 15- ment. Small individuals 20 to 40 

 ^' ^ ^' millimeters in length have been found 



with matured sexual cells, while larger specimens were often immature. The 

 best fixing agent was found to be sublimate acetic. It caused little rupture of 

 tissues and less shrinkage than fluids containing picric acid, after which the 

 epithelium was frequently torn so as to render the material worthless. The 

 regions containing the ovaries were cut away with scalpel upon hardened liver, 

 stained, cleared, and studied in toto prior to sectioning. Double staining in 

 borax-carmine and Delafield's haematoxylin was employed. Nuclei took the car- 

 mine stain, while blood, yolk, and membranes were stained by the haematoxylin. 



c. A. K. 



Kotte, E. Beitrage zur Kenntniss der Haut- The author describes in this paper the 



sinnesorgane und des peripheren Nerven- ,1111 1 j ■ j 



systems der Tiefsee-Decrpoden. Zool. Jahrb. method he has employed in demon- 

 Abth. f. Anat. u. Ont. 17: 619-659, Taf. 23- stating the sense organs of some fresh 

 "''' '^°-^" \i2Xtx Crustacea. Only customary stain- 



ing methods were used on the material with which the paper deals. Fresh water 



