-534 Journal of Applied Microscopy 



forms such as Cyclops^ Branchipus, Daphnia, Gammarus, and Asellus were placed 

 for 1 to 2 days in a faintly colored solution of methylen blue. Without exception 

 they live in such solutions without disaster. After removal from the solution 

 they were washed in water and examined under cover glass. In Daphnia the 

 antennal sense organs, the ventral nerve chain, and the post abdominal sense 

 organs are clearly demonstrated by this method. Attempts to apply the Golgi 

 method were fruitless. c. a. k. 



Qoldschmidt, R. Histologische Untersuchun- The parasites were taken directly from 



gen an Nematoden, I, Die Sinnesoigane ^, . ^ ,. , ,, , , , , , . 



von Ascaris lumhrkoides L. und A. megalo- the intestine of the host and placed in 



cephala. Cloqu. Zool. Jahrb. Abth. f. Anat. the fixing fluids. Histological preser- 

 u. Ontog. 18: 1-57, Taf. 1-5, 1903. .. ,, , ^. ^,. , 



^ vation was poor (because of the thick 



caticula) if the entire animal was placed in the fluid. It was therefore desirable 

 to cut the worms into small pieces. This is contrary to the experience of Apathy, 

 who recommends fixing the entire worm in hot sublimate. The author finds, 

 however, that the nemofibrillse are best demonstrated in material which is other- 

 wise in a state of poor histological preservation. Best results were obtained 

 with sublimate-acetic (sublimate y^ cone. + 2 per cent, acetic), and the follow- 

 ing were found useful : concentrated sublimate, Perenyi's fluid, and acetic-alco- 

 hol (70 per cent. ale. 4 parts -]- 1 part 43 percent, acetic acid). Picro-sulphuric, 

 picro-acetic, chrom-acetic, and Hermann's fluid gave poor results. Chloroform- 

 paraffin method of infiltration was employed and sections 2 to 6// in thickness 

 were used. The sense organs of the anterior end were best studied in frontal 

 sections. In toto staining with Heidenhain's chrome-ha;matoxylin gave a very 

 intensive and at the same time transparent stain. Pieces of tissue were stained 

 for 12 hours in yi per cent, aqueous haematoxylin followed by 1 per cent, 

 chromate of potash for an equal length of time. Delafield's haematoxylin 

 followed by eosin gave excellent detail, as did also Van Gieson's method. 

 Apathy's gilding method and methylen blue intra vitani were tried without suc- 

 cess. C. A. K. 



Weber, A. L'origine des glandes annexes de Embryos Studied were those of Aliniop- 



li^T^'l' V^T ""q^" ^^' ''T'^''^'- ^''''^- ^erus se/ireit>ersii fixed for several days 

 d Anat. Micr. 5: 455-727, pis. 17-27, 1903. _ _ ■' 



in bichromate-acetic, of Anguis fragilis 

 fixed in sublimate acetic or in Zenker's fluid, and of the duck fixed in various 

 sublimate mixtures — Carnoy's, Zenker's, and sublimate-acetic. Duck embryos 

 fixed in these sublimate mixtures are quickly hardened and exhibit a minimum of 

 folding or dislocation of parts. For advanced stages, after formation of the umbil- 

 ical region, fixing fluids based on bichromate of potash, such as bichromate-acetic 

 or Zenker's fluid, preserve the organs in proper relations. To obviate the difficul- 

 ties arising from sublimate in Zenker's fluid the author places embryos of 5 to 6 

 days incubation in this fixing agent for 12 hours only, then without washing 

 placed them directly in bichromate-acetic for several days, changing the fluid 

 several times. In this way the sojourn of these large embryos in iodine-alcohol 

 is materially reduced. Embryos intended for use in graphic or plastic recon- 

 struction were overstained in borax carmine or Mayer's alcoholic carmine. 



Outlines of embryonic organs such as the notochord or neural tube were 



