2574 Journal of Applied Microscopy 



2. Study the hygroscopic movements of the teeth of the peristome by gently 

 breathing on the slide while making observations. Describe the movements. 

 Of what use ? 



3. Study the true stomata on the hypophysis. Make a sketch of the cap- 

 sule, showing the peristoine and the hypophysis with stomata. The hypophysis 

 may be compared with a leaf of the higher plants. 



4. Cut open a capsule longitudinally and mount. Examine under high 

 power and draw a stoma, showing the two guard cells and some of the surround- 

 ing cells. Draw several of the teeth of the peristome and some of the non- 

 sexual spores. How many teeth are there ? Are they in one or two circles ? 



5. Cut a cross section of a green capsule, mount and examine under low 

 power. Sketch the section, noting the following structures : epidermis, hypo- 

 dermal parenchyma, air space, spore sac, central columella. 



(b) Aulacomniutn palustre (L.) Schw. 

 Order, Bryales. Family, Bryaceae. 



This moss is common in boggy ground and may be found on charred logs 

 and stumps or on the ground. Collect the material and grow in a moist 

 chamber. 



1. Under dissecting microscope make a sketch of a stem, modified in the 

 upper part, the scales of which are easily detached. These scales act as brood 

 buds, and when they fall to the ground are able to develop a protonema. 



2. Mount some of the detached scales from near the tip and draw under 

 low power. 



(c) Leptflbryum pyriforme (L.) Wils. Long-necked Bryum. 

 Family, Bryaceae. 



This interesting little moss may be found on moist, shaded cliffs and on 

 rocks near water. It is very abundant in greenhouses, where the gametophyte 

 may be obtained at any season. The rhizoids contain peculiar tuber-like brood 

 buds of a dark-brown color. On the young sterile fronds these tuber-like bodies 

 are often numerous, being produced on short rhizoids which come from the 

 axils of the scales. 



1. Mount and draw a mature brood-bud under high power, showing the 

 structure. Describe. 



2. Draw a rhizoid with an enlarged, light-colored end cell, the incept of the 

 brood-bud. 



A STUDY OF FORMS WHICH FORESHADOW SOME OF THE STRUCTURES 

 DEVELOPED IN THE FOLLOWING SUB-KINGDOM. 



LII. Splachmim ampullaceiivi L. 



Class, Musci. Order, Bryales. Family, Splachnaceas. 



Although this odd looking moss is not very common, an attempt should be 

 made to obtain fresh or alcoholic material of specimens containing mature or 

 nearly mature sporophytes. The plant grows on decaying animal tissue or 

 excreta and is said to occur in cranberry swamps from Ohio to New Jersey and 

 northward. 



