•2586 Journal of Applied Microscopy 



fermentation of dough and finds this a different phenomenon. The fermentation 

 is in this case not produced by yeasts but by two species of bacteria both of 

 which produce gas. h. w. c. 



Klopstock. Beitrag zur Differenzieiung von This work tests and expands the method 

 Typhus, Coli und Ruhrbacillen. Ber. klin. ^f differentiating typhoid bacilU first 

 Woch. 803, 1903. , ,,-o., o., . ,j 



advanced by Barsickow. This method 



uses two media, each containing nutrose, salt, and water and either milk sugar 

 or glucose. Klopstock combines the two and obtains a medium which he re- 

 gards as satisfactory in separating typhoid, colon, and dysentery bacilli. By 

 putting both milk sugar and glucose into a medium composed of nutrose and 

 salt, and inoculating with these different bacilli in fermentation tubes he deter- 

 mines the following diognostic characters. The dysentery bacillus produces 

 simply an acid reaction. The typhoid bacillus produces an acid reaction and a 

 curdling, while the colon bacillus produces acid, curdles, and forms gas. 



H. w. c. 



Behrend. Nachprufung zwei neuer Methoden The author compares the methods of 

 der Geisselfarbung bei bakterien. Hyg. flagella Staining devised by Rossi and 

 Hund. 691, 1903. , r^. ; 



Peppier. Theformer uses as a mordant 



concentrated tannin solution and as a stain Ziehl's solution diluted. This stain 

 gave unsatisfactory results, as Behrend believes, because of the weak action of 

 the mordant. The method of Peppier uses a mordant of saturated tannin, to 

 which is added 2.5 per cent. HCl, and as a stain carbol gentian-violet. The use 

 of this mordant for three minutes, followed by the stain for two minutes, gives 

 excellent results. He finds the most favorable objects for staining are eight to 

 thirteen hour cultures. h. w. c. 



Cambier. Note sur une nouvelle methode de The author adds another to the numer- 

 recherche du bacille d' Erberth. Rev. ^^^ methods of separating the typhoid 

 d'Hyg. 64, 1902. , .,, . . , . ., 



bacillus from B. coh and other similar 



organisms. It depends upon the difference in rapidity with which the organ- 

 isms will grow through a porcelain filter. He prepares a solution of 



1000 c. c. 3 per cent, solution of peptone 



80-100 c. c. 1 per cent. NaOH 



88-120 c. c. saturated salt solution. 

 The suspected material, e.g., feces, in solution is placed inside the candle of 

 a Chamberland filter F., and the lower part of the candle placed in the solution 

 above described. The whole apparatus is incubated at 37°, and, if typhoid 

 bacilli are present, the bouillion becomes cloudy in a few hours and is found to 

 contain nearly a pure culture of typhoid bacillus. 



For testing water he first filters many liters through a similar filter, and then 

 places the filter in the solution in the same manner. h. w. c. 



