and Laboratory Methods. 2631 



CURRENT ZOOLOGICAL LITERATURE. 



CHARLES A. KOFOID, University of California. 



Books and Separates of Papers on Zoological Subjects should be Sent for Review to Charles A. 

 Kofoid, University of California, Berkeley, California. 



i 



<:x,„.„, r. I TT u J- ^r A J The author suggests a procedure which 



Streeter, u. L. Ueber die Verwendung der _ &&> t' 



Faraftineinbettung bei Markscheidenfar obviates the necessity of preparing sec- 



bung. Arch. f. mik. Anat. 62: 734-740, tions by the celloidin method in stain- 

 1903. -^ 



ing the myelin sheath of nerve fibres 



by the Weigert method. He takes advantage of the fact that haematoxylin forms 

 a compound insoluble in xylol or parafBn when brought in contact with chrom- 

 mordanted myelin. Fresh material is mordanted at room temperature for 4 to 8 

 days in mixture of 5 per cent, potassium bichromate and 2 per cent, fiuorchrom. 

 The slower acting (2 to 3 months) 5 per cent, potassium bichromate, or Muller's 

 fluid may be used to even greater advantage by reason of more uniform penetra- 

 tion. Formol and copper acetate are to be avoided because of interference with 

 subsequent staining. The fixing agent is washed out very thoroughly in 80 per 

 cent, alcohol changed frequently for 2 to 3 weeks or in running water. Long 

 exposure to alcohol is no detriment. The preparations are then stained /// fo/o 

 in Weigert's lithium carbonate-haematoxylin, the stain being renewed at 24 and 72 

 hours. After washing for 48 hours in 70 per cent, alcohol the objects are 

 embedded in paraffin by either the chloroform or xylol method, the usual pro- 

 cedure being followed. For small animals sections 10 to 15 // in thickness are 

 to be preferred. To prevent possible loss of sections from the slide a thin coat 

 of celloidin is spread over them after dipping the slide in alcohol-ether. Sec- 

 tions were differentiated in Weigert's ferricyanide of potash (2^^ per cent.) — 

 borax (2 per cent.) solution diluted ten times, or with better results by Pal's 

 method, washed for 48 hours in running water and by the usual procedure — 

 alcohols, carbo-xylol, and xylol — carried into balsam. The results are most 

 satisfactory in economy of time, perfection of series and completeness of the stain- 

 ing. Experiments are in progress in quest of a stronger stain with greater 

 capacity of penetration. c. a. k. 



^ ^ ^ ^ „. , . , „. , Rabbit ovaries taken from 20 1 2 hours 



Conn, r. Zur Histologic und Histogenese des . 



Corpus luteumunddesititerstitiellenOvarial- tO 15 days />0S^ CoitUPl were fixed in 



gewebes. Arch. f. mik. Anat. 62 : 745-772, Tellyesniczky's acetic-bichrcmate or 



Taf. 31 und 8 figs, im Text, 1903. • i 1 r» 



in Zenker's fluid. Sections 4 to 10 /^ 

 in thickness were stained in haematoxylin-eosin, in Mallory's phospho-tungstic 

 acid-haematoxylin, in Heidenhain's iron-hasematoxylin, or in Rabinovic's haema- 

 toxylin devised for nerve staining. This last stain was especially favorable for 

 the demonstration of the structure of the protoplasm. Mallory's connective 

 tissue stain was used to demonstrate the thecal connective tissue in the corpus 

 luteum. ^- A. K. 



