2706 Journal of Applied Microscopy 



CURRENT ZOOLOGICAL LITERATURE. 



CHARLES A. KOFOID, University of California. 



Books and Separates of Papers on Zoological Subjects should be Sent for Review to Charles A. 

 Kofoid, University of California, Berlieley, California. 



,.,.,,,, . ., .. Material for study was secured from 



Drzweeki, W. Ueber vegetative \ organgc im -' 



Kern und Plasma der Gregarina des Regen- the testis of the earthworm (Lumbricics 

 wurmhodens Arch. f. Protistenkunde 3: agricola) in March-Tulv and fixed in 

 107-126, Taf. 9, I o, 1903. * ' ... 



picro-acetic acid mixture or in a mix- 

 ture of one-half per cent, each of chromic and osmic acids combined with 

 Perenyi's fluid in the proportions of 2:3. Living material was examined in the 

 blood of the earthworm giving excellent opportunities for observing nuclear 

 degeneration. Borax carmine and Delafield's ha;matoxylin were used as stains 

 and most of the results were obtained from material thus stained, teased and 

 mounted in clove oil or thin glycerine. c. a. k. 



^ , ., ^ ., The organism formed an intense green 



Steuer, A. Ueber eine Euglenoide [Entreptia] " 



aus dem Canale grande von Triest. Arch. water-bloom in the salt water of the 



f. Protistenkunde 3: 126-138,0111 13 Text- grand canal on June oU-July 1, con- 



figuren, 1903. ° . • i 1 



stitutmg a temporary monotonic plank- 

 ton. Numbers decreased rapidly after July 2, and the species diappeared by 

 July 13. Material was examined in the living condition, and also fixed in sub- 

 limate and in Flemming's solution, stained in Grenacher's or in iron htematoxy- 

 lin and mounted in glycerine, clove oil or balsam. c. a. k. 



D, . „ w ^ ^ . , . ■ • „„,. ,„ This parasite of the blood plasma was 



PlehO, M. Trypanoplasnia cyprtm nov. sp. -^ " 1 1 



Arch. f. Protistenkunde 3: 175-181, Taf. found in the German carp in a few 



'"' '9°3- individuals, often abundantly and at 



times only sparingly. Carp kept in aquaria and inoculated with the disease 

 become anaemic when badly infested and the gills become pale. In extreme 

 cases only a few drops of a watery scarcely reddish blood can be obtained from 

 the host. 



Streak preparations of the fresh blood which is infested will show a dozen or 

 more parasites in a single field of the immersion lens, but owing to the rapid 

 motion of the flagellates no observations can be made on their structure. As 

 the preparation slowly dries up the motion slackens and the moribund individ- 

 uals afford the only means of examining the living specimens. The parasite is 

 very resistant, living for a day and continuing its movements in the body 

 of the host, in normal salt solution or in a cover-glass preparation, if kept 

 from drying up, and they have been known to live even for two days in a neutral 

 red solution. The flagella do not stain in this dye, and the nucleus takes only 

 a faint tinge at death. Vital staining with methylen blue was very successful, 

 but the parasites are killed by it in about 30 minutes. The flagella stain deeply 

 in this dye and are readily demonstrated by allowing the stain to act for a few 

 minutes and then fixing the preparation in fumes of osmic acid for a few seconds. 



