and Laboratory Methods. 2707 



These preparations do not reveal the nucleus, are best examined in water and 

 may be subsequently stained. One method of staining proved successful. Dry 

 preparations fixed for 1.5 minutes in absolute alchol were stained in Romanow- 

 sky-Ziemann's methylen blue-eosin. The nucleus takes a deep blue-violet color 

 and the so-called centrosome appears as a mass of reddish granules. The dry- 

 ing process destroys the small flagellum and distorts the form of the body. 



c. A. K. 



Bezzenberger, E. Ueber Infusorien aus asia- Material for this Study was secured 

 tischen Anuren. Arch, f . Protistenkunde 3 : from living ^////;vr and was examined 

 1 ^S-171;, Taf. 1 1 und 21 Textfiguren, igo-j. .... .... 1 1 , 



■^ ^ in living condition in normal salt solu- 



tion, fixed in Schandinn's sublimate-alcohol mixture which likewise fastens the 

 animals to the substratum, slide or cover-glass. Picro-carmine was used as a 

 stain for /;; toto preparations. Portions of the intestine and its contents were 

 sectioned in 5 yu sections and stained in paracarmine or haematoxylin-eosin. 

 Sections 1 to 2 /< in thickness stained in iron-haematoxylin were used for fine 

 details of structure. The foreign matter and intestinal mucus was removed from 

 Opa/ina raiianim by shaking the intestinal contents vigorously in a tube with 

 normal salt solution and then removing the isolated parasites to fresh fluid in 

 which they were again shaken. They were finally rid of the foreign matter by 

 allowing them to filtrate through a loose-meshed wad placed a few centimeters 

 above the bottom of a cylinder of water. Intra 7'itam staining was accomplished 

 successfully by the use of moderately strong solution of Romanowsky's soda 

 methylen blue prepared after the method of Ruge for the malarial parasites. 

 Not all of these parasitic infusoria could be stained in this fashion, some died 

 quickly in the stain and other species resisted its action. c. a. k. 



Stevens, N. M. Further Studies on the Ciliate Conjugating individuals of Lkhnophora 

 Infusoria, Lichnophora and Boveria. Arch. found upon their hostS Thysanozoon, 



f. Protistenkunde 3 : 1-44, Taf. 1-6, iqoi. ^ , • ^ ^ , i ■ ,i ■ 1 1 1 



^ ■^ Asterina and Ophwthnx, when placed 



upon a glass slide attach themselves to the glass and remain in the one place 

 until the gametes separate. Tke process of conjugation continues for more than 

 17 hours. It was found to be possible to fasten these conjugating pairs to the 

 slide for subsequent treatment in staining, etc., by fixing them in absolute alco- 

 hol -j- 5 percent, glacial acetic acid. Fixing fluids containing osmic acid caused 

 Lichnophora to loosen its hold upon the glass and corrosive-acetic permitted 

 them to fall off in the alcohol. Maupas's glycerine methods were not success- 

 ful with this form, and the micro nucleus was difficult to stain in anything but 

 iron-haeniatoxylin in sections. Whole material was stained for 12 hours in alum 

 carmine and decolored in acid alcohol. c. a. k. 



^ . ^ . ^ . , ^ . . Trypanosoma Ieiuisiocc\\xs\x\ the blood 



Francis, h. An Experimental Investigation 

 of Trypanosoma lewisi. Bull. No. n, Hyg. of the wild gray rat of Europe and the 



Lab. U. S. Pub Health & Mar. Hosp. Serv. gg^g^ j.^. y^^^ ^^^^ ^^^ {oyi.x\A in ratS 

 Wash., pp. 1-26, 4 pis. 1903. 



captured in Washington. White and 



spotted rats were inoculated and the parasite was studied in fresh and stained 

 preparations of the blood. Fresh blood in the hanging drop at room tempera- 

 ture and at 37° C. though carefully examined failed to yield any other stages of 



