2708 Journal of Applied Microscopy 



the parasite than those present when the blood was first drawn. Examinations 

 of blood drawn at short intervals from a rat after intraperitoneal inoculation will 

 provide the entire cycle of development. In white rats the infection lasts from 

 seven to fourteen days. The ordinary anilin and ha^matoxylin stains fail com- 

 pletely in staining Trypanosoma, but the Romanowsky, the Goldhorn, and the 

 Tenner stains all gave most excellent results. Wright's modification of the 

 Romanowsky method was employed as follows : One per cent, methylene blue 

 is added to 'j per cent, solution of sodium bicarbonate and the mixture is 

 steamed in an Arnold sterilizer for one hour. When cold, eosin is added till the 

 color changes from blue to purple and a metallic scum is formed on the surface. 

 The black precipitate which forms is collected on a filter, dried, and dissolved 

 in methyl alcohol. The blood films spread evenly, are dried in the air and are 

 then covered with the alcoholic solution of the dye for one minute. Water is 

 then added drop by drop to the preparation until the dye becomes semitranslu- 

 cent and a yellowish metallic scum forms on the surface. This mixture remains 

 on the preparation for 2 to 3 minutes. The preparation is then washed in dis- 

 tilled water until the film has a yellowish or pinkish tinge, is dried between filter 

 papers and mounted in balsam. c. a. k. 



„ , £. T., ,, ,• . , Mastis:a7)Mha,Cerco7nonas.Monas,^\co- 



Prowazek, S. Flagellatenstudien, Anhang ; 



Fibiillare Struciuren der Vorticellinen. SOeca, Chilomonas, Polytoma, and Vor- 



Arch. f. Protistenkunde 2: 195-212, Taf. 5, ^^^^\\^ were fixed in quantity in Flem- 



6, 1903. ^ ■" 



ming's fluid, condensed by the use of 

 a Cori's hand centrifuge, and by its help carried through the usual technique 

 preliminary to embedding in paraffin. The concentrated material was brought 

 from xylol into a trough made in a watch glass full of cold paraffin and then 

 placed in a warming oven and embedded. Sections :2 to 3 // in thickness were 

 stained in iron htematoxylin. C. A. k. 



Taylor, J. R. Observations on the Mosquitos This paper contains a number of SUg- 

 of Havana, Cuba. Reprint from La Revista gestions of value to those who wish to 



Med. Trop., June, 1903, 27 pp. v f *u r * j 



^ . V J. / i-j- rear mosquitos for the purpose of Study. 



Eggs deposited at night usually hatch out early in the day so that search for 

 them should be made in early morning hours. A white enamelled ladle or 

 dipper is very useful in examining the margins of pools and other bodies of 

 water, for eggs, larvae, or pupae. Larvae may be raised in the laboratory in small 

 aquaria supplied with debris from the bottom of the breeding pools. A small 

 amount of raw rice or bread may also be added to increase to amount of food in 

 the aquaria, but care must be taken to prevent fouling or stagnation, by chang- 

 ing the water when necessary. When the pupae appear they should be changed 

 to small dishes and then placed in the gauze covered breeding cages. Large 

 and airy breeding cages should be provided if eggs are desired from captive 

 females. Copulation occurs soon after emergence from the pupal stage, and 

 most captured females have been fertilized and will lay fertile eggs if provided 

 with a meal of blood. For this purpose the hand may be introduced into the 

 breeding cage, or a pigeon or rabbit may be placed in the cage during the sleep- 

 ing hours of the animal. The eggs are laid within a day or two after the meal. 



