538 Journal of Agricultural Research voi. x, No. 10 



were successful. They included (i) four deep slit inoculations with 

 agar cultures of Epicoccum sp. with corresponding number of controls. 

 This species of Epicoccum produced roundish to ellipsoid, dark, olivaceous, 

 continuous spores, with reticulated surfaces, and in addition short, 

 blunt protuberances. The long diameter of these spores varies from 

 20 to 28 jj.. The form obtained agrees with E. granulatum Penzig de- 

 scribed upon the twigs of Acer pseudoplatarms Kinn.* 



(2) Eight deep slit inoculations with a species of Phyllosticta and four 

 controls made in the same manner. This form was found commonly 

 associated with the Epicoccum sp. upon blighted branchlets, together 

 with Alternaria sp. and Pestalozzia funerca Desm. It is distinct from 

 the species of Phoma that causes the red-cedar blight, and produces 

 a globose, uniformly thin- walled, black pycnidium opening by a very 

 definite pore through which a mass of spores ooze from a single chamber. 

 These spores are ovoid to ellipsoid, nonguttate, 4.5 to 8 by 2.5 to 4 ix. 

 Since this form was found only upon the needles rather than on the 

 stems, it is assigned to Phyllosticta instead of Phoma. Alternaria and 

 Pestalozzia, though occurring commonly, were not tested. Inoculations 

 with Pestalozzia spp. are now in progress. 



Inoculations were also made with the parasitic Phoma sp. on hosts 

 other than Junipcrus virginiana as follows : In the greenhouse on Septem- 

 ber 25, 1 91 6, deep slit inoculations were made upon young potted plants 

 of Thuja occidentalis E. and T. orientalis E. and upon /. barbadensis E. and 

 /. pachyphloea Torr. The mycelium of single-spore cultures was used. 

 All of these inoculations were successful. With the T. occidentalis 

 there was a tendency for the blight to become limited and the lesions to 

 callus over, but with T. orientalis in every case (eight inoculations) the 

 stem was girdled and the upper part killed (PI. 60, B). Upon the killed 

 parts of the T. orientalis placed under a bell jar the pycnidiaand character- 

 istic spores of Phoma sp. were produced. 



Eater tests upon a wider variety of hosts include successful inoculations, 

 with single spore cultures freshly isolated, upon Juniperus communis E., 

 /. communis sibirica (Burgsd.) Rydberg, /. prostrata Pers., Cupressus 

 glabra Sudworth, and Thuja plicata Don. Negative results were obtained 

 upon Libocedrus decurrens Torr. and Chamaecyparis lawsoniana (Murr.) 

 Pari. Whether the Phoma sp. will give positive results upon the eastern 

 species of Chamaecyparis has not been determined. 



CONTROL EXPERIMENTS 



Spraying, the most promising preventive measure, has been tested 

 by a commercial nurseryman thus far with rather uncertain results. 

 The fungicides used were commercial lime-sulphur solution and Bordeaux 



1 LiNDAU, Gustav. FUNGI imperfecti: hyphomycetes. p. 599. Leipzig, 1909. (Rabenhorst, Ludwig. 

 Kryptogamen-Flora von Deutschland . . . Aufl. 2, Bd. i, Abt. 9, Lfg. 114-) 



