Oct. i, 1920 An Experimental Study of Echinacea Therapy 69 



the right thighs. On the following day 0.5 mil of the remedy was in- 

 jected into the left thighs of the animals. This treatment was wholly 

 remedial, no protective doses having been given as in experiments 1 and 

 2 of this series. In three days after the injection of the toxin all the 

 animals were dead after exhibiting typical tetanus. The autopsy pic- 

 ture was similar to that in experiment 2. 



EXPERIMENT 4. — INULA AND ECHINACEA INJECTED INTRAMUSCULARLY 



Protective doses of the "Subculoyd Inula and Echinacea" were in- 

 jected intramuscularly into five guinea pigs. The dose administered 

 was 0.5 mil per day for six days, a total of 3 mils, corresponding to 1 

 gm. of echinacea and 1.33 gm. of inula. On the eighth day after the 

 treatment was begun the animals were injected with 0.5 mil of tetanus 

 toxin solution, and a dose of 0.5 mil "Subculoyd" was given. The 

 total dose of the remedy was 3.5 mils. On the following day all the 

 guinea pigs showed typical symptoms of tetanus, and one died; the 

 remaining four died the next day. On autopsy there was found a mod- 

 erately severe local reaction of the tissues to the injection of the inula 

 and echinacea. The subcutaneous and muscular tissues at the site of 

 injection showed considerable hemorrhage and sero-sanguineous infiltra- 

 tion. No gross lesions were apparent in any of the internal organs. 



EXPERIMENT 5. — INFLUENCE OF ALCOHOL ON TETANUS 



Since the "Specific Medicine Echinacea" employed in the foregoing 

 experiments contained 69 per cent of ethyl alcohol, it was considered 

 desirable to study the influence of this factor upon tetanus under the 

 conditions of the echinacea experiments. Accordingly, a mixture of 

 alcohol and distilled water was made which contained exactly 69 per 

 cent of alcohol, and this was injected intramuscularly into four guinea 

 pigs in 0.5-mil doses. Each guinea pig received two doses, one into the 

 right thigh and, on the next day, one into the left thigh. Two days 

 afterwards all four received 0.5 mil of tetanus toxin solution subcuta- 

 neously. In three days two of these animals died, and the remaining 

 two died during the following night. All showed typical symptoms of 

 tetanus. The autopsy showed some congestion of the subcutaneous 

 tissues at the points of injection of the alcohol, hemorrhage in the mus- 

 culature, and evidence of local degeneration of the muscles. No gross 

 lesions were apparent in any of the internal organs. 



EXPERIMENT 6. — CONTROLS 



The six control animals were kept under the same conditions as the 

 experimental animals and received the same amounts of tetanus toxin. 

 They all developed the typical symptoms of tetanus and died in less 



