Oct. i, 1920 An Experimental Study of Echinacea Therapy 73 



EXPERIMENT 2. — ECHINACEA ADMINISTERED PER OS (REMEDIAL) 



Two guinea pigs were given two daily doses per os of 3 mils of the 

 diluted echinacea mixture used in experiment 1. On the third day they 

 were injected with 0.5 mil of Bacillus bovisepticus culture, and imme- 

 diately afterwards were given a 3-mil dose of the echinacea mixture per 

 os. On the following day both animals were very sick. They were 

 given a fourth dose of 3 mils of the echinacea mixture per os. The total 

 dose was 4 mils of specific echinacea, equal to 4 gm. of the root. Case 65 

 died in 24 hours and case 66 in 48 hours. The autopsy showed septicemia ; 

 typical organisms were demonstrated in blood and organs. 



EXPERIMENT 3. — INULA AND ECHINACEA INJECTED INTRAMUSCULARLY (PROTECTIVE) 



This experiment was conducted exactly like experiment 1 of this 

 series except that the "Subculoyd" preparation was used instead of 

 the "Specific Medicine Echinacea." Three guinea pigs were given four 

 daily doses of the "Subculoyd" preparation, 0.5 mil being injected 

 intramuscularly, first into the right and then into the left thigh. The 

 total dose was 2 mils. The animals were allowed to rest 1 1 days and 

 then were injected subcutaneously with 0.5 mil of Bacillus bovisepticus 

 culture. All became sick. Case 62 died in 6 days after th° inoculation, 

 case 64 died in 12 days, and case 63 survived, being discharged as re- 

 covered 10 weeks after the injection. The autopsies on the fatal cases 

 revealed typical pictures of septicemia, and the organisms were demon- 

 strated in the blood and organs. 



EXPERIMENT 4. — INULA AND ECHINACEA INJECTED INTRAMUSCULARLY (REMEDIAL) 



Three guinea pigs were given daily injections of the "Subculoyd" 

 preparation, the injections being made alternately into the right and 

 left thighs. The dose used was 0.5 mil. After the third injection the 

 animals were all inoculated subcutaneously with 0.5 mil of Bacillus 

 bovisepticus culture. On the following day the animals were given a 

 fourth dose of the "Subculoyd." The total dose of remedy was 2 mils. 

 All these cases succumbed to the infection, the first in one day, the second 

 in two days, and the third in three days after the inoculation. The 

 autopsies showed the typical septicemia pictures, and the organisms 

 were demonstrated in the blood. 



EXPERIMENT 5. — CONTROLS 



Three control animals, each inoculated subcutaneously with 0.5 mil 

 of Bacillus bovisepticus culture, all became sick, and two succumbed to 

 the infection. The third survived and after 10 weeks' observation was 

 discharged as recovered. The autopsies on the fatal cases showed 

 septicemia, and the organisms were demonstrated in the blood and organs. 



The experiments for septicemia are summarized in Table III. 

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