78 Journal of Agricultural Research voi.xx.No.i 



EXPERIMENT I. — ECHINACEA ADMINISTERED PER OS 



Three guinea pigs were inoculated with tubercle bacilli November 20, 

 1919, and on the following day treatment was begun. Each animal re- 

 ceived a dose of a mixture of 0.25 mil of fluid extract echinacea and 0.75 

 mil distilled water per os each week day. The animals were weighed 

 three times a week. All the animals showed a progressive loss in weight 

 (see Table VII) and eventually succumbed. Case 85 died December 10, 

 (20 days after inoculation), after having received a total of 3.5 mils of 

 fluid extract echinacea as a remedy. The autopsy in this case was nega- 

 tive. Case 84 died December 22 (32 days after inoculation), having 

 received 6 mils of the echinacea. Case 86 was found dead in the morning 

 of December 26 (36 days after inoculation), having received 6.75 mils of 

 the echinacea. In the last two cases the autopsies revealed the typical 

 picture of generalized tuberculosis. 



As these were probably the first experimental animals which had ever 

 received echinacea over an extended period of time, it was interesting 

 to observe the effects of the plant on the animals themselves, and espe- 

 cially upon the gastrointestinal tract. In case 85 there was found a 

 chronic catarrhal gastritis which was absent in cases 83 and 84. Apart 

 from the tubercular lesions there was no abnormality found in the other 

 organs upon macroscopic examination. 



EXPERIMENT 2. — INULA AND ECHINACEA INJECTED SUBCUTANEOUSLY 



Three guinea pigs were inoculated with the tuberculous material as 

 in experiment 1 on November 20, 191 9, and the treatment was begun on 

 the following day. Each animal received subcutaneously 0.2 mil of 

 the "Subculoyd Inula and Echinacea" each week day and was weighed 

 three times a week. All showed progressive loss of weight, as shown 

 in Table VII. Case 87 died December 19, 29 days after inoculation, 

 after having received 4.2 mils of the remedy. Case 88 died December 

 23, 33 days after inoculation, having received 5 mils of the remedy, and 

 case 86 died on December 28, 38 days after inoculation, having received 

 5.8 mils of the remedy. The autopsies in these cases showed great 

 emaciation, some necrosis at the points of injection, and generalized 

 tuberculosis. There was no evidence of systemic effects from the 

 remedy. 



EXPERIMENT 3. — CONTROLS 



Two control guinea pigs were inoculated with the same tuberculous 

 material as the animals in experiments 1 and 2, on November 20, 191 9, 

 and were weighed three times a week. They lost weight (see Table 

 VII). Case 89 died December 3, 13 days after inoculation, and case 

 90 died December 23, 33 days after inoculation. The autopsy showed 

 generalized tuberculosis. 



The experiments are summarized in Table VI. 



