94 



Journal of Agricultural Research 



Vol. XX, No. 2 



Table VII. — Comparative germicidal efficiency of chloramin T, Dakin's solution, 

 eusol, and mercuric chlorid against Staphylococcus aureus, Bacillus pyocyaneus, and 

 B. typhosus in the presence of 25 per cent, blood serum a 



EXPERIMENT 14 



EXPERIMENT 15 



Chloramin T: 



1 to 1,000 



1 to 2,000 



Dakin 's solution : 



NaOCl 1 to 2,000. . . . 



NaOCl 1 to 4,000. . . . 

 Eusol: 



HOCI 1 to 2,000 



HOCI 1 to 4,000. . . . 

 Mercuric chlorid: 



1 to 2,000 



1 to 4,000 



+ 



+ 



+ 



+ 



+ 



+ 

 + 



+ 

 + 



+ 



+ 



+ 



+ 



+ 



+ 



+ 

 + 



+ 



+ 



+ 



a 4- signifies growth; — no growth. 



The four experiments shown in Tables VI and VII show that eusol is 

 decidedly superior to chloramin T, Dakin's solution, and mercuric chlorid, 

 especially in the presence of blood serum. Unfortunately, however, 

 eusol is very unstable and for that reason is not reliable, since it is im- 

 possible in practice to count on its containing any uniform amount of 

 active material. In the tests reported here the eusol was made up just 

 before the test and was used as soon as possible, but observations which 

 were made in connection with chemical work upon these various disin- 

 fectants would tend to show that there was probably a perceptible loss 

 of strength even in the time required for a test. 



EXPERIMENTS WITH ANTHRAX SPORES 



The experiments upon anthrax spores were performed by the Hill (6) 

 "rod" method, with some modifications. The method as modified is 

 as follows: Glass rods 3 /i 6 - m ch in diameter and 8 inches long are etched 

 at one end, the etched portion being about 1 inch long. Cotton is wrapped 



