104 



Journal of Agricultural Research 



Vol. XX, No. 2 



The experiments shown above in Table XVI indicate that the addition 

 of a molecular equivalent of ammonia to Dakin's solution not only greatly 

 increases its germicidal value against "naked" bacteria, but, to a large 

 extent, prevents depreciation of germicidal value due to the addition 

 of blood serum. 



In Table XVII there are shown the results of a number of experiments 

 upon chlorin water, with and without a molecular equivalent of ammonia. 



Table XVII. — Effect of ammonia upon the germicidal activity of chlorin in aqueous 

 solution against Bacillus typhosus a 



EXPERIMENT 29 



WITHOUT AMMONIA 



Concentration of chlorin. 



I to 4,000 



I to 8,000 



I to 12,000 

 i to 16,000 

 Phenol 1 to 80 



Ex- 

 posed 



2K 



min- 

 utes. 



+ 



Ex- 

 posed 



5 



min- 

 utes. 



+ 

 + 



Ex- j Ex- 

 posed posed 



min- 

 utes. 



+ 



min- 

 utes. 



Ex- 

 posed 

 12% 

 min- 

 utes. 



Ex- 

 posed 



IS 

 min- 

 utes. 



WITH AMMONIA 



1 to 4,000 



1 to 8,000 



1 to 12,000 

 1 to 16,000 

 Phenol 1 to 80 



EXPERIMENT 30 



WITHOUT AMMONIA; IO PER CENT BLOOD SERUM ADDED 



i to 500 



I to I.OOO 



I to 2,000 



1 to 4,000 



Phenol 1 to 80 b 



WITH AMMONIA; IO PER CENT BLOOD SERUM ADDED 



I to 1,000 



I to 2,000 



1 to 4,000 



I to 6,000 



Phenol 1 to 80 b 



a + signifies growth; — , no growth. 



h No blood serum added. 



