io6 



Journal of Agricultural Research 



Vol. XX. No. 2 



Table XVIII. — Effect of varying amounts of ammonia upon the germicidal value of 

 chlorin in aqueous solution — Continued 



EXPERIMENT 33 



WITH ONE-HALF MOLECULAR EQUIVALENT OP AMMONIA 



Concentration of chlorin. 



I to 10,000 



i to 15,000 



I to 20,000 



i to 25,000 



Phenol 1 to 80. 



Ex- 

 posed 



min- 

 utes. 



+ 



WITH ONE-FOURTH MOLECULAR EQUIVALENT OF AMMONIA 



I to 10,000 — 



1 to 15,000 + 



I to 20,000 4" 



1 to 25,000 + 



Phenol 1 to 80 + 



+ 

 + 

 + 

 + 



+ 

 + 



+ 



+ 

 + 



+ 



+ 



+ 

 + 



+ 

 + 



EXPERIMENT 34 



WITH MOLECULAR EQUIVALENT OF AMMONIA; IO PER CENT BLOOD SERUM ADDED 



1 to 1,000 + 



1 to 2,000 + + + + + + 



1 to 4,000 4- + 4- 4- 



1 to 6,000 + 



Phenol 1 to 80 + 



WITH ONE-HALF MOLECULAR EQUIVALENT OP AMMONIA; IO PER CENT BLOOD SERUM ADDED 



I to 1,000 



1 to 2,000 



1 to 4,000 



I to 6,000 



Phenol 1 to 80. 



The experiments shown in Table XVIII indicate that the optimum 

 amount of ammonia is approximately one-half of the molecular 

 equivalent. 



Experiments were next made with anthrax spores, using the following 

 method: Equal quantities (2% cc. each) of chlorin solution and spore 

 suspension, with or without blood serum added to it, were mixed in a 

 test tube and vigorously shaken. After it had stood at room temperature 

 for the required time of exposure the mixture was again shaken, and a 

 subculture was made by a standard platinum loop into a tube of nutrient 

 broth. No attempt was made to neutralize any excess of disinfectant. 

 The results of these experiments are shown in Table XIX. 



