Oct. 15, 1920 Germicidal Value of Some Chlorin Disinfectants 



107 



Table XIX. — Germicidal activity of chlorin against anthrax spores with and without 



addition of ammonia ° 



EXPERIMENT 35 



without ammonia; 10 per cent blood serum added 



WITH ONE-HALF MOLECULAR EQUIVALENT OF AMMONIA; io PER CENT BLOOD SERUM ADDED 



i to 1,000. 

 i to 2,000. 

 i to 4,000. 



Number of spores 350,000, or 

 70,000 per cubic centimeter. 



Experiment 36 



WITHOUT AMMONIA; 10 PER CENT BLOOD SERUM ADDED 



WITH ONE-HALF MOLECULAR EQUIVALENT OF AMMONIA; IO PER CENT BLOOD SERUM ADDED 



1 to 2,000. 

 1 to 4,000. 

 1 to 6,000. 

 i to 8,000. 



Number of spores 350,000, or 

 70,000 per cubic centimeter. 



a + signifies growth; — , no growth. 



The results shown in Table XIX seemed to show clearly that chlorin 

 with ammonia added had very great germicidal value, even in the 

 presence of organic matter in the form of blood serum. 



Experiments were, therefore, undertaken to ascertain whether or not 

 such a solution could be used for disinfecting hides. The technic was 

 as follows: Small pieces of dry hide, cut to the same weight, were infected 

 by soaking them in a suspension of anthrax spores and then drying 

 them over sulphuric acid in a vacuum equal to about 5 mm. of 

 mercury for 48 hours. These pieces of infected hide were then treated 

 with the disinfectant solution in the proportion of 5 times as much 

 solution as hide by weight. At the end of the required period of ex- 

 posure the pieces of hide were transferred to a solution of sodium thio- 

 sulphate of sufficient strength to neutralize completely the disinfectant 

 carried over by the hide. After neutralization the hair and more or 



