io8 



Journal of Agricultural Research 



Vol. XX, No. a 



less of the hide surface were scraped off with sterile instruments and 

 plated, using exactly neutral agar. The results of two such experiments 

 are given in Table XX. 



It should be noted in this connection that the stock solutions of 

 chlorin water and Dakin's solution from which test dilutions were pre- 

 pared by the machine devised by the Wallace & Tiernan Co., of New 

 York, for the preparation of Dakin's solution, chlorin being run directly 

 into water or a solution of sodium carbonate, as the case might be. 



Table XX. — Germicidal activity of chlorin water and Dakin's solution against anthrax 



spores on pieces of hide 



EXPERIMENT 37 



CHLORIN WATER WITH MOLECULAR EQUIVALENT OF AMMONIA 



CHLORIN WATER WITH ONE-HALF MOLECULAR EQUIVALENT OF AMMONIA 



i to 500. . 

 I to 1,000 

 I to 2,000 



Plates over- 

 grown. 

 ....do 



.do. 



Colonies too 



many to count. 



...do 



.do. 



80 colonies, 70 

 anthrax. 



Plates over- 

 grown. 



do 



20 anthrax col- 

 onies. 



40 anthrax col- 

 onies. 



Colonies too 

 many to count. 



° No count possible on account of spreaders. 

 EXPERIMENT 38 



DAKIN'S SOLUTION WITH NO AMMONIA ADDED 



Concentra- 

 tion of 

 available 

 chlorin. 



Exposed 6 hours. 



Exposed 18 hours. 



Exposed 24 hours 



Exposed 48 hours. 



I to 250. 

 I to 500. 



/Plates all show heavy growth, spreaders plus discrete and confluent 

 \ colonies of anthrax. 



DAKTN S SOLUTION WITH ONE-HALF MOLECULAR EQUIVALENT OF AMMONIA 



i to 250. . . 

 i to 500. . . 



1 colony ° 



Many anthrax 

 colonies. 6 



10 colonies 



4 colonies, 2 an- 

 thrax. 



1 colony a 



No colonies vis- 

 ible, c 



4 colonies. 

 2 colonies. 



DAKIN S SOLUTION WITH MOLECULAR EQUIVALENT OF AMMONIA 



1 to 250. . 

 1 to 500. . 



Many anthrax 



colonies. 6 

 do 



4 colonies. 



1 colony an- 

 thrax. 



1 colony ° . 



No colonies vis- 

 ible, c 



4 colonies. 

 1 colony. 



o Not anthrax. 



6 No count possible on account of spreaders. 



c Covered by spreaders. 



