Feb. 15, 1921 



Amylase of Rhizopus tritici 



781 



Experiment 3. — In the following experiments, Czapeck's nutrient 

 solution was used for series a, b, and c, and sweet potato bouillon was 

 used for d, the reducing sugars (glucose in b and c) and starch being 

 determined in the solutions before and after the growth of the fungus. 

 The digestion period was 12 days at 35 ° C. 



In these experiments no account is taken of the amount used by the 

 fungus or that converted to other compounds by it. 



The digestive power of the mycelium was determined by using 0.2 

 gm. enzym powder in 100 cc. of a 0.5 per cent starch paste solution, 

 which was hydrolyzed for 18 hours at 40 C. (Table XV.) 



Table XV. — Amount of reducing sugars and starch in solutions before and after the 

 growth of the fungus; also the hydrolysis of starch by enzym powder 



[Expressed in milligrams per 10 cc. of solution] 



These results accord in general with those of the previous experiments, 

 series a and d having the greatest hydrolyzing power and b and c the 

 least. 



Experiment 4. — The foregoing experiment was repeated, the solu- 

 tions being made to contain roughly the same amount of glucose and 

 starch. The hydrolysis of starch by the enzym powder was determined 

 by using 0.25 gm. enzym powder in 100 cc. of a 0.5 per cent starch paste 

 solution and hydrolyzing 18 hours at 40 C. (Table XVI.) 



Table XVI. — Amount of reducing sugars and starch in the solutions before and after 

 the growth of the fungus; also the products of hydrolysis of starch by enzym powder 



[Expressed in milligrams per 10 cc. of solution] 



