12 



Journal of Agricultural Research 



Vol. VI, Xo. I 



Table XI. — Effect of carbon bisulphid on the growth cf red clover in acid soils 



No. 



I 

 3 



3 



4 



5 

 6 



7 

 8 



9 

 lo 

 II 



12 

 13 

 14 



IS 



16 



Soil. 



Miami silt loam . 



do 



do 



do 



Sparta sand . . . . 



do 



do... 



do 



Colby silt 



....do 



do 



do 



Peat 



do 



do 



do 



Carboo 



bisulphid 



added. 



Per cent. 



Control. 

 Control. 



Control. 

 Control. 



Control. 

 Control. 



Control. 

 Control. 



Weight of first crop, clover. 



Green. 



Gni. 



138 

 140 



158 

 124 



36 



33 



19 



31 



95 



87 



153 



^33 



4 



2 



83 

 79 



Dry. 



Gm. 



(a) 



(«) 

 (a) 



(«) 



Average 

 green. 



Gm. 

 139 

 141 



34 

 25 

 91 

 143 

 3 

 81 



Weight of second crop, 

 clover. 



Green. 



Gm. 

 129 



145 

 168 



131 



58 



, 48 



18 



: 43 



no 

 85 



108 



82 



6 



53 

 46 



Dry. 



Gm. 

 19 



21 

 26 

 20 



13 

 ID 



4 



8 

 20 

 14 



15 

 12 



2.8 



2 



9 



8-5 



Aver- 

 age. 



Gtn. 



■ 20 



• 23 



• II 



■ 6 



• 17 



■ 13 



• 2.4 



• 8.7 



a Lost. 



Plate II, figure 2, shows the relative growth of clover in the treated 

 and untreated soils. 



Each figure for Miami silt loam in Table XI represent^ the average of 

 triplicate jars. Because of the individual variation, it was decided to use 

 12 jars for this experiment. Six of these were used as controls and six 

 treated with 2 per cent of carbon bisulphid. It is evident from the data 

 that medium red clover in Miami soil is benefited both in the first and 

 second crop by the antiseptic. In the Sparta sand a decrease was noted 

 with each crop. The Colby silt loam gave a decided increase with the 

 first crop, but not with the second. 



Previous tests with these soils showed that the clover bacteria were 

 present in sufficient numbers to produce good inoculation. In view of the 

 large amount of carbon bisulphid applied, it was thought that this sub- 

 stance would probably injure nodule formation. However, examination 

 of the root systems showed this was not the case. The plant roots were 

 thoroughly inoculated, both in the treated and tmtreated soils. Appar- 

 ently the plants in carbon bisulphid soils contained the greater number 

 of nodules. 



Because of the remarkable action of carbon bisulphid in peat soil, this 

 part of the previous test was repeated. In addition to carbon bisulphid, 

 the effect of flowers of sulphur was studied. If the data in the previous 

 experiment are correct, the carbon bisulphid should greatly increase the 

 growth of clover. A glance at the results in Table XII confirms this 

 statement. 



