Apr. 17. i9i6 Two New Nematodes 119 



size that about four to five side by side would span the bodj-^ diameter. Numerous 

 micro-organisms \vere seen in the intestine. 



Male formula. Yi 3^1 3*5 3 9 09 ^'^^ ™"^' v^^"?'^ specimen). The tail of 



the male diflfers m'aterially in form from that of the female. It begins to taper at the 

 anus, and it tapers rapidly in the anterior two-thirds, more particularly in the middle 

 third, so that at the beginning of the final third it is only about one-tenth as wide 

 as at the anus. Thence onward it tapers rather regularly to the exceedingly fine ter- 

 minus; there is, however, a pronounced ventral elevation at the beginning of the small 

 part of the tail, though it remains uncertain whether this elevation is innervated. 

 The middle portion of the tail is strongly convex-conoid , the convexity existing largely 

 on the dorsal side. The cuticle of the tail presents a peculiar arrangement of the dots, 

 such that there is an appearance of two sets of oblique fibers crossing each other, these 

 fibers being arranged approximately at 45° to the longitudinal lines. The two equal, 

 rather uniform, somewhat arcuate, blunt spicula are about one and one-fourth to one 

 and one-half times as long as the anal body diameter. Their proximal ends, which 

 are slightly narrower than the main portion, are set off by a rather broad and prominent 

 constriction. At their widest part, through the middle, they are about one-fifth to one- 

 sixth as wide as the corresponding portion of the body. The accessory piece is about 

 half as long as the spicula. It is very inconspicuous near the anus, but lies parallel 

 to the spicula. It widens out to a somewhat clavate or elongated pyriform contour, 

 and has its roimded proximal end toward the dorsal side of the body, and from this 

 blunt end muscular fibers pass obliquely backward to the ventral surface of the tail 

 and join the caudal wall at a distance nearly half way from the anus to the beginning 

 of the narrow portion. Oblique copulatory muscles are to be seen opposite the ejacu- 

 latory duct for a distance about one and one-half times as great as the length of the 

 tail. The male papillae are arranged as follows: One ventrally submedian pair a little 

 in front of the proximal ends of the spicula; one ventrally submedian pair a little in 

 front of the anus, and one ventrally sublateral pair on the same zone; another sub- 

 lateral pair just opposite the anus; a lateral pair slightly behind the middle of the 

 enlarged portion of the tail ; a submedian pair nearly halfway from that last mentioned 

 to the beginning of the small part of the tail; a dorsally sublateral pair a little in 

 front of the beginning of the narrow portion of the tail ; three subventral pairs close 

 together opposite that last mentioned; between the members of these three sub- 

 ventral pairs, possibly a single ventral papilla. The most pronounced of these 

 papillae can hardly be called digitate. The ejaculator}' ducc is about two-fifths as 

 wide as the body. The vas deferens is nearly two-thirds as wide as the body. The 

 testis tapers so that at the point of inflection, a short distance behind the cardiac bulb, 

 it is about one-fourth as wide as the body. The blind end lies about two body widths 

 behind the flexure. 



Habitat: Manhattan, Kans., 1915, on Saperda tridentata. 



The eggs of Diplogaster labiata, elliptical in shape, about twice as long 

 as wide, with bluntly rounded ends, when freshly deposited, were uni- 

 formly dark brown or gray, but after segmentation began they became 

 darker. Their average length was 0.0627 mm. and the average diameter 

 0.031 mm. They were laid singly with apparently no preference as to 

 the place of deposition. Occasionally segmentation began before the 

 eggs were deposited. From the beginning of segmentation the cell divi- 

 sions could be plainly followed throughout (fig. 2, A-H). 



A few hours before emerging, the folded young nematodes made 

 slight movements within the egg. Later these movements became 



