May 15, 1916 Cold Storage and Pupce of Mediterranean Fruit Fly 259 



pleted their development and produced adults in storage on the fifth 

 day of refrigeration, and that thereafter emergence of adults continued 

 until all living pupae yielded adults in storage by the end of the sixteenth 

 day of refrigeration except 2, which yielded adults on the fourth day 

 after removal after 16 days of storage. Data on 1,900 eight-day-old 

 pupae show that from i to 5 pupae among each of 14 different lots of 100 

 completed their development and yielded adults on the second day of 

 refrigeration, that an average of 43.5 per cent of 14 lots yielded adults 

 on the third day, and that emergence of adults was completed by the 

 seventh day except in one instance where 2 pupae yielded adults in storage 

 on the ninth and tenth days of refrigeration. 



Table VII. — The effect upon six-day-old Mediterranean fruit-fly pupce of refrigeration at 

 54° to 57° F. Pupce placed in refrigeration August 22, IQIJ 



Data on file covering observations on 1^600, 1,400, 1,700, 2,200, 3,300, 

 4,000, 3,100, 3,500, and 1,900 pupae, i, 2, 3, 4, 5, 6, 7, 8, and 9 days old, 

 respectively, show a steady increase in the pupae completing their devel- 

 opment and yielding adults in refrigeration, and their tabulation shows 

 a transition from the condition in Table VI through that of Table VII 

 to the condition set forth for eight-day-old pupae. One lot of two-day-old 

 pupae left in storage for 37 days yielded adults almost daily between the 

 twenty-sixth and thirty-fifth days of refrigeration. Eight lots of three- 

 day-old pupae left in storage from 32 to 39 days yielded adults between the 

 twenty-third and thirty-third days of refrigeration and none thereafter. 

 Fourteen lots of four-day-old pupae in storage from 25 to 39 days yielded 

 adults between the sixteenth and twenty-seventh days of refrigeration 

 and none thereafter. Eight lots of seven-day-old pupae in storage from 

 13 to 21 days yielded adults between the second and eleventh days of 

 refrigeration and none thereafter. 



