May 29. 1916 Clear and Sterilized Anti-Hog-Cholera Serum 



337 



from such defibrinated blood by the use of bean extract and sodium 

 chlorid, and of the cell residues from which the clear serum was removed. 

 In preparing the cells for injection they were taken up in distilled water 

 and made to a volume' corresponding to the volume of defibrinated 

 blood from which they were derived. Thus hog 2149 received all of the 

 cell residue from 200 c. c. of defibrinated blood and hog 2150 received 

 all of the cell material from 100 c. c. of defibrinated blood. The serum 

 which was obtained from the defibrinated blood was used to inoculate 

 hogs 2155 to 2158, inclusive. 



Table II. — Test of serurn separated by use of bean extract and sodiujn chlorid in igido- 



" No inflammation or swelling at point of injection on any pigs in this test. Thriftiness of pigs remain- 

 ing normal not impaired. 



From the fact that both of the pigs injected with the cell material 

 contracted hog cholera and died it seems clear that, in this experiment 

 at least, the amount of antibodies left behind with the cells was negligible. 



The bean-extract-sodium-chlorid method of separating the corpuscles 

 from defibrinated hogs' blood has been applied repeatedly in these labora- 

 tories and always with success. There seems to be no reason why the 

 process should not be entirely satisfactory for use in the practical pro- 

 duction of anti-hog-cholera serum. There appears to be little or no loss 

 in antibodies; the serum secured is generally clear; and it may be re- 

 moved from the agglutinated cells easily by pouring from the cups. 

 The method also would seem to tend toward a certain concentration of 



