564 Journal of Agricultural Research vol. vi, no. 15 



a day or two by spraying with sterile water from an atomizer. Cultures 

 from beef bouillon were used for all inoculations. 



On November 27 eight tubers were inoculated with organism 3616 

 from a 3-day-old culture and all were completely decayed in seven days. 

 The organism was recovered from four. Strain 3616 was isolated from 

 a Pat-long-fu taro {Colocasia esculenta) on November 18, 191 5. The 

 conn was decayed at the base and was lifted a few days before in the con- 

 dition shown by Plate LXXXIII. Ten raw sterile blocks inoculated on 

 the same day with each of strains 3595 and 3616 were completely decayed 

 in three days. On November 24 ten tubers were inoculated with organ- 

 ism 3624 {Bacillus carotovorus from Dr. L. R. Jones) from a 24-hour-old 

 culture. A slight rot had taken place in seven days; and in 12 days, 

 although the decay had increased, it was still slight. The causal organ- 

 ism was recovered from three tubers. On December 1 eight tubers were 

 inoculated with strain 3624, and in five days about half of each tuber was 

 decayed. The organism was recovered from four. The checks, six in 

 number, remained sound. On December 9 six raw carrot and six raw 

 turnip blocks in test tubes were inoculated with strains 3616 and 3595. 

 Decay started in 24 hours and was complete in five days. At the same 

 time thirteen raw blocks of dasheens were inoculated with strain 3624 from 

 a 2 -day-old culture, and in 5 days nine blocks were completely decayed; 

 the others and the ten checks remained sound. On December 13 three 

 turnips and three carrots in moist chambers were inoculated with a 6-day- 

 old culture of strain 3595, and by December 20 two turnips and one carrot 

 were completely decayed. At the same time six dasheen tubers were 

 inoculated with a 6-day-old culture of strain 3624. Decay began in 

 24 hours and in seven days had destroyed most of each tuber. The four 

 checks remained sound. On December 20, 191 5, four turnips and four 

 carrots were inoculated with a 7-day-old culture of organism 3627 (a 

 reisolation of 3616) and three turnips and four carrots with strain 3595. 

 A rot started in 48 hours and decay was complete in seven days. 

 The causal organism was recovered from the four turnips inoculated 

 with strain 3627 and from the four carrots inoculated with strain 3595. 

 The ten checks remained sound. 



On December 27 four dasheens were inoculated with 1 -day-old cul- 

 tures of strain 3616 and six carrots and seven turnips with strain 3624. 

 By January 3 the dasheens were nearly decayed and all the turnips and 

 five of the carrots completely rotted. All the checks remained sound. 

 Eleven turnips inoculated with 1 -day-old cultures of strain 3616 were 

 completely decayed by January 3, 1916. Four dasheen corms inoculated 

 with 1 -day-old culture of 3624 were but slightly rotted at the end of 

 seven days. On December 28 three turnips and four carrots were 

 inoculated with a 24-hour-old culture of organism 3626 (an isolation 

 from a fibrovascular bundle at the center of a large conn). In six days 



