Oct. 14, 19 18 



Chemistry of Sweet-Clover Silage 



129 



was determined both by the electrometric and the colorimetric methods. 

 The results from the Van Slyke method were very much larger, but we 

 did not investigate the reasons for this. To make a thorough compari- 

 son of the Van Slyke method with the formol-titration method would 

 probably require as extended an investigation as the one reported in 

 this paper. 



Table VIII. — Comparison of amount of titrable nitrogen stated as milligrams per 5 gm, 

 of silage in alcoholic and water extracts, colorimetric titration 



Age of silage. 



Days 



O 



I 



2 



3 



4 



6 



7 



8 



10 



13 



15 



17 



21 , 



29 



43 



64 



98 



Alfalfa alone. 



Sweet clover alone. 



Water. 



1-7 



4. I 

 12.5 



12. 9 

 13.0 

 13.0 



13-7 

 16. 7 

 16. 9 

 12. 4 



15-1 

 21. 2 

 24. 2 

 22.8 

 20. 7 

 24. 2 

 26.6 



Alcohol. 



4-5 

 10. 9 

 13.6 



13- 7 

 13.0 



13-9 

 14.4 



17-3 

 17. 2 

 17. 6 

 17.9 

 18.3 

 17.9 



21. 4 



22. I 

 23-5 



2-5 



9-5 

 10.5 

 12.3 

 12. I 



9.6 



"■3 

 12. 4 

 12. 2 

 12.8 

 12.8 

 18.9 



19-3 

 16. 7 

 16. 7 



1S.7 



2.9 

 5-7 

 7-9 

 8.5 

 10. 4 



9.8 



10. 2 



11. 8 

 II. 9 



13-1 

 13.0 

 13. 2 



15-5 

 15.0 



16. 5 



Sweet clover and com 

 meal. 



Water. 



1-3 

 6.9 



8.4 



9-5 

 lo. 4 



9-7 

 12.8 



15-3 

 13-3 

 14. 2 

 16.3 

 20. 2 

 16. 4 

 19.7 

 19. o 



Alcohol. 



10. 2 

 9.8 



11. 6 

 II. 6 



13- I 

 14-5 

 14. I 

 15.6 



13-4 

 16. I 



TOTAL, STUTZER'S, OR ALBUMINOID,AND AMID NITROGEN IN SILAGE, 



KJELDAHL METHOD 



The total and Stutzer's nitrogen, sometimes called "albuminoid 

 nitrogen," were determined on the samples from the moisture deter- 

 minations. The result stated as milligrams per 5 gm. of silage are 

 given in Table IX. 



The results given in Table IX show that — 



(i) In silage from alfalfa alone the nitrogen in amid ^ form is approxi- 

 mately one-half of the total. In silage from sweet clover alone it is a 

 little more than one-half of the total, and when com meal is added, the 

 proportion is somewhat smaller. 



1 The term "amid" is used simply to designate the difference between the total and albuminoid nitrogen 

 asdeterminedby Stutzer's method (Wiley, H. W., ED. official and provisional methods of anal- 

 ysis, ASSOCIATION OF official agricultural chemists, as compiled by the committeb on revision 

 OF methods. U. S. Dept. Agri. Bur. Chem. Bui. 107 (rev.), p, 38. 1908. Reprinted, 191a. 



