I30 



Journal of Agricultural Research 



Vol. XV. No. 2 



(2) The nitrogen in amid form as determined by this method does 

 not show the same degree of progressive increase as the nitrogen deter- 

 mined by titration, as shown in Table VIII. The drying of the sample 

 may have something to do with this. That the process of drying may 

 influence the form of nitrogen in forage material is shown by investi- 

 gations now in progress at this laboratory. 



(3) The amount of nitrogen in amid form in matured silage is a little 

 larger as determined by Stutzer's method than by the formaldehyde 

 titration method, but the differences are not large. Compare Tables VI 

 and VII with Table IX. 



Table IX. — Total nitrogen, Stutzer's nitrogen, and amid nitrogen in silage 

 [Results are expressed as milligrams per s gms. of silage.] 



Age of silage. 



Alfalfa alone. 



Total 

 nitro- 

 gen. 



Stut- 

 zer's 

 nitro- 

 gen. 



Amid 

 nitro- 

 gen. 



Sweet clover alone. 



Total 

 nitro- 

 gen. 



Stut- 

 zer's 



nitro- 

 gen. 



Amid 

 nitro- 

 gen. 



Sweet clover and corn 

 meal. 



Total 

 nitro- 

 gen. 



Stut- 

 zer's 

 nitro- 

 gen. 



Amid 

 nitro- 

 gen. 



Days. 



I 



2 



3 



4 



6 



7 



8 



10 



13 



15 



17 



21 



29 



43 



64 



29. o 



29-5 

 32.0 

 29. o 



25.0 



26. o 



26.5 



25-5 

 24- 5 

 22.5 

 24. o 



27-5 

 26.5 

 30.0 



25-5 

 24. o 



29. o 



27. o 



28.0 

 29-5 



45- 5 

 26. 5 

 23.0 

 26. 5 



27-5 

 26. o 

 28.0 



26. o 



32-5 



24- 5 

 28.5 



27. o 



33-5 

 36.0 



36.5 

 36-5 



35-5 



21.5 

 22. o 

 20. o 

 20. o 

 19-5 



12. o 

 14. o 



16.5 

 16.5 



16. o 



42. 

 41. 

 40. 



35- 

 43- 



27. o 

 26.5 

 25-5 



24-5 



26. o 



35- o 

 33-5 

 32-5 

 30. o 



37- o 

 36. o 

 34- o 

 34- o 



29- 5 

 32.0 



19. o 

 16.5 



15-5 

 15.0 

 16.5 

 15.0 

 14. o 

 16. o 

 14. o 

 14. o 



16. o 



17. o 

 17. o 



15.0 



20. 5 



21. o 

 20. o 



18.0 



IS- 5 

 18.0 



38.0 

 39-5 

 42.5 

 41-5 

 40.5 

 41. o 



39- o 

 35-5 

 35-5 

 37- o 



23-5 

 23-5 

 24. o 

 23.0 

 21.5 

 22. o 



21.5 

 19-5 



20. o 

 19. o 



15.0 

 15.0 

 14.5 



lo-S 

 17. o 



14-5 

 16. o 

 18.5 

 18.5 

 19. o 

 19. o 



17-5 

 16. o 



15-5 

 18.0 



NITROGEN IN WATER AND ALCOHOLIC EXTRACTS OF SILAGE, 

 KJELDAHL METHOD 



The water and alcoholic extracts of silage were used for the deter- 

 mination of nitrogen, Kjeldahl method, both total and that not precipi- 

 tated by phosphotungstic acid. The results stated in milligrams per 

 5 grams of silage are given in Table X. 



The results given in Table X show that — 



(i) The water and the alcoholic extracts gave practically the same 

 amount of total nitrogen, showing that the solvent action of 50 per sent 

 alcohol is not different from that of water. 



