Oct. 14, 1918 



Chemistry of Sweet-Clover Silage 



131 



(2) The soluble nitrogen is approximately two-thirds of the total in the 

 silage made from alfalfa alone and from sweet clover alone. When com 

 meal was added to sweet clover, the proportion of water-soluble to total 

 nitrogen was a little more than one-half. Compare Table IX with 

 Table X. 



(3) In the water extract the nitrogen not precipitated by phospho- 

 tungstic acid is approximately three-fourths of the total soluble nitrogen. 

 In the alcoholic extract the nitrogen precipitated by phosphotungstic 

 acid is practically the same in the amount as the total soluble in 50 per 

 cent alcohol. 



(4) Thus, while the total solubility is not much different in the water 

 and alcoholic extracts, the nature of the solution is different as measured 

 by the precipitating action of phosphotungstic acid. This is either due 

 to the solubility of the protein phosphotungstate in the alcohol or to the 

 fact that the ionization is such that the precipitate will not form when 

 the phosphotungstic acid is added. 



Table X. — Total nitrogen and nitrogen not precipitated by phosphotungstic acid in water 

 and alcoholic extracts of silage 



[Results expressed as milligrams of nitrogen from 5 gm. of silage.] 



Age of silage. 



Alfalfa alone. 



Water 

 extract. 



Total 

 nitro 

 gen. 



Nitro- 

 gen 

 not 

 pre- 

 cipi- 

 tated 

 by 

 phos- 

 pho- 

 tung- 

 stic 

 acid 



Alcoholic 

 extract. 



Total 

 nitro- 

 gen. 



Nitro- 

 gen 

 not 

 pre- 

 cipi- 

 tated 

 by 

 phos- 

 pho- 

 tung- 

 stic 

 acid. 



Sweet clover alone. 



Water 

 extract. 



Total 

 nitro- 

 gen 



Nitro- 

 gen 

 not 

 pre- 

 cipi- 

 tated 

 by 

 phos- 

 pho- 

 tung 

 Stic 

 acid. 



Alcoholic 

 extract. 



Total 

 nitro- 

 gen 



Nitro- 

 gen 

 not 

 pre- 

 cipi- 

 tated 

 by 

 nhos- 

 pho- 

 tung- 

 stic 

 acid. 



Sweet clover and com 

 meal. 



Water 

 extract. 



Total 

 nitro- 

 gen. 



Nitro- 

 gen 

 not 

 pre- 

 cipi- 

 tated 

 by 

 phos- 

 pho- 

 tung 

 Stic 

 acid. 



Alcoholic 

 extract. 



Total 

 nitro- 

 gen 



Nitro- 

 gen 

 not 

 pre- 

 cipi- 

 tated 

 by 

 phos- 

 pho- 

 tung- 

 stic 

 acid. 



Days 



o 



I 



2 



3 



4 



6 



7 



8 



10 



13 



IS 



17 



21 



29 



« 



64 



98 



26.0 

 30.0 

 29.0 

 27.5 

 26.5 

 30.0 

 30-5 



30- 5 

 29. o 

 31-0 

 34- o 

 3i- 5 

 35-0 

 3-So 

 37-0 

 34-5 



20. o 

 16. s 



19. o 

 23- S 



24.5 

 24-5 



28. s 

 27.5 



23.0 

 26.0 

 28.0 

 26.5 

 29. o 



26.0 



29- S 

 30-0 

 31-0 

 30- o 



36. S 



3»-o 



36- S 



24.5 

 28.0 



29- 5 



28.5 



30-5 

 28.0 

 31-0 

 31-0 

 34- o 

 310 

 32-5 

 32-0 

 3(>-5 

 3I-S 

 42. o 

 38.0 



7.0 

 19.0 



7-5 

 19.0 

 20. o 

 18. s 



10.5 

 12.0 



14- S 

 13- S 



IS- 5 

 15.0 

 18.0 

 20.0 

 17- S 



14- S 

 16.5 

 18.5 

 20. 5 

 18.0 



II. o 

 17.0 

 16.0 

 18.0 

 I7-0 

 20.0 



4-S 

 9.0 



II. o 



10. 5 

 12.0 

 iS-o 



16.5 

 17.0 

 18.0 

 19.0 



2I-S 



14. s 

 IS-S 

 16.0 

 19.0 

 19.0 



IS-O 



21. o 



21. O 



31-5 



22. 5 

 23.0 

 22. 5 

 21.0 

 20. s 



14. o 

 17-S 

 15- 5 

 17-S 

 16- S 

 16. o 

 17-5 

 16. o 

 MS 



12. 5 



18.0 

 20.0 



2I-S 



20. 5 



21. O 

 22.0 



22. 5 

 2I-S 

 22. S 

 25.0 



19. O 



26. 5 

 34.0 



19- 5 

 20. 5 

 22.0 

 22.5 

 21.5 

 23-S 



23-0 

 24.0 



14. o 

 14- S 

 lb. o 

 I7-0 

 16.5 



16. o 

 18.0 

 17-S 



17. o 



19- S 



21- S 



26. o 

 36. o 



19- S 



20. O 

 22. S 

 22. S 

 32. 5 

 25.0 

 34.0 

 24.5 



26. s 

 36. s 



