144 



Journal of Agricultural Research 



Vol. XV, No. 3 



Table III. — Effect of the reaction of the dioxogen upon the catalase activity of various 

 seeds; also the buffer effect of the seed material 



[0.9 cc. of Njio sodium hydroxid neutralizes 25 cc. of dioxogen to phenolphthalein. 

 for clover; 70-niesh for other seeds.] 



loo-mesh bolting cloth 



with loo-mesh and very nearly maximum with 200-mesh, but much 

 reduced activity with 40-mesh. Agitation of enzyms generally causes 

 degeneration. The fall in activity with finer pulverization may be due 

 to the denaturing of the catalase by excessive mechanical manipulation 

 in grinding and working the powder through the very fine bolting cloth. 



Table IV. — Effect of the degree of pulverizing upon catalase activity 



[Bolting cloth used: 70, 80, 100, and 200 mesh to the inch. The sieve used had circular holes 0.610 mm. in 



diameter] 



o o.6io-mm. holes. 



