Dec. 9, 1918 Seedling Diseases of Conifers 



533 



REISOLATION AND RElNOCUtATION 



To complete the proof of the parasitism of Corticium vagum reisola- 

 tions were made from recently killed seedlings of jack and western yellow 

 pine in pots which had been inoculated with the fungus in an experiment 

 in which the controls had remained free from disease. The results in 

 the initial experiment and in two subsequent experiments conducted 

 at different times in which the reisolated strains were used are given in 

 Table I. 



Table I. — Inoculation ivith initial and reisolated strains of Corticium- vagum on pine 



seedlings in autoclaved soil 



Experi- 

 ment 



No. and 

 date. 



Strain 

 No. 



Sotirce. 



Trial host. 



Nvim- 



ber 



of 



pots. 



lyOcation of in- 

 oculum. 



Results. 



val. 



58, fall, 

 1915- 



Do.. 

 Do.. 



Do.. 



Do.. 



Do.. 



71 and 



72, fall, 



1917. 



Do... 



Do... 

 Do... 

 Do... 



Do... 

 Do... 



Do... 



Do... 



Do... 

 Do... 



Do... 



...do.. 

 «*I3 



230 

 Control 

 ...do 



333 

 343 



a 213 

 340 



341 

 Control 



Damped -off Engel- 

 mann spruce seed- 

 lings. 



do 



Damped-off sugar- 

 beet seedlings. 



Russian wild-olive 

 seedlings. *> 



Damped - oS Engel- 

 mann spruce seed- 

 lings. 



Reisolation of No. 147 

 from western yel- 

 low pine, experi- 

 ment 58. 



....do 



.do. 



Reisolation of No. 147 

 from jack pine, ex- 

 periment 58. 



Damped-ofI sugar- 

 beet seedlings. 



Reisolation of No. 213 

 from jack pine, ex- 

 periment 58. 



Russian wild-olive 

 seedlings 6 (dupli- 

 cate of No. 230). 



Reisolation of No. 230 

 from jack pine, ex- 

 periment 58. 



....do.... 



Western yel- 

 low pine. 



Jack pine. . . . 

 do 



do 



Western yel- 

 low pine. 

 Jack pine . . . . 



Western yel- 

 low pine. 



.do. 



.do. 



.do. 

 .do. 



.do. 

 .do. 



.do. 



.do. 



.do. 

 .do. 



Agar cultures at i 

 point per pot. 



do 



Agar cultures at 6 



points per pot. 

 Agar cultures at 8 



points per pot. 

 None 



do 



Agar cultures; frag- 

 ments scattered 

 over one side of 

 pot. 



....do 



.do. 

 .do. 

 .do. 



.do. 

 .do. 



.do. 



.do. 



.do. 



Sterile agar frag- 

 ments scattered 

 over one side of 

 pot. 



Agar culture frag- 

 ments scattered 

 over one side of 

 pot. 



a Cultures furnished by Dr. H. A. Edson, Bureau of Plant Industry. 

 6 Diseased material furnished by Mr. B. R. H. d'AUemand. 



Damped - off Engel- 

 mann spruce seed- 

 lings. 



Red pine. 



Per s 



pots. 



55 

 78 



Per 3 



pots. 



9 



16. s 



