Dec. i6, i9i8 Bacteriological Studies on Alfalfa Silage 591 



CONCLUSIONS 



(i) Silage made from alfalfa alone is of an inferior quality. By the 

 addition of an available carbohydrate supplement, a good quality of 

 silage may be produced. 



(2) The microbial flora of the two types of silage, so far as noted, was 

 practically identical. 



(3) The chemical data demonstrate that alfalfa with a supplement 

 produced a higher acid content than alfalfa alone. 



(4) Alfalfa silage shows a greater amount of protein decomposition, as 

 evidenced by the amino-nitrogen and ammonia determinations. 



(5) The decomposition of protein was checked by the addition of a 

 utilizable carbohydrate. 



LITERATURE CITED 



(i) Bertrand, Gabriel, and Weisweiller, Gustav. 



1907. EINWIRKUNG DES BUtGARISCHEN FERMENTES AUF DIE MILCH. In Liebig's 



Ann. Chem., Bd. 351, p. 486-503. 



(2) Hart, E. B., Hastings, E. G., Funt, E. M., and Evans, Alice C. 



I914. RELATION OP THE ACTION OF CERTAIN BACTERIA TO THE RIPENING OF 



CHEESE OF THE CHEDDAR TYPE. In Jouf. Agf. Research, v. 2, no. 3, 

 p. 193-216. Literature cited, p. 214-216. 



(3) Hastings, E. G., Evans, Alice C, and Hart, E. B. 



I912. STUDIES ON THE FACTORS CONCERNED IN THE RIPENING OF CHEDDAR 



CHEESE. In Wis. Agr. Exp. Sta. Research Bui. 25, 54 p., 6 fig. Also 

 published as U. S. Dept. Agr. Bur. Anim. Indus. Bui. 150, 1912, under 

 the title. The bacteria of Cheddar cheese. 



(4) HeinEmann, p. G., and Hefferan, Mary. 



1909. a study op baclllus bulgaricus. In Jour. Infect. Diseases, v. 6, no. 

 p. 304-318, 5 fig. 



(5) Hunter, O. W. 



I917. microorganisms and HEAT PRODUCTION IN SILAGE FERMENTATION. 



In Jour. Agr. Research, v. 10. no. 2, p. 75-83, ro fig. Literature cited, 

 p. 82-83. 



(6) and BushnELL, L. D. 



1916. SOME IMPORTANT FERMENTATIONS IN SILAGE. KanS. Agr. Exp. Sta. 



Tech. Bui. 2, 1,2 p. Bibliography, p. 32. 



(7) Kendall, Arthur I. 



191 1. THE BIOLOGY AND BIOCHEMISTRY OF BACTERIA AND THEIR RELATION TO 



THERAPEUTICS. In Jour. Med. Research, v. 24 (n. s. v. 19), no. 2, 

 p. 411-424. 



(8) and Farmer, Chester J. 



1912. STUDIES IN bacterial METABOLISM, i-iii, V, VII. luJouT. Biol. Chem., 



V. 12, no. I, p. 13-21; no. 2, p. 215-218; no. 3, p. 465-471; v. 13, no i, 

 p. 63-70. 

 (9) Bagg. Edward P., jr., and Day, Alexander A. 



1912. STUDIES IN B.vcTERiAL METABOLISM. IV. In Jour. Biol. Chem., V. 12, 



no. 2, p. 219-221. 

 (10) Day, Alexander A., and Walker, Arthur W. 



1913. STUDIES IN bacterial METABOLISM, xiii-xxx. In Jour. Amer. Chem. 



Soc, V. 35, no. 9, p. 1201-1249. 



