Nov. 15. 1915 Gossypol, the Toxic Substance in Cottonseed Meal 263 



These products, differing in purity, have been designated by us as "gossy- 

 pol extract," "precipitated gossypol," and "gossypol 'acetate.'" All 

 proved toxic to rabbits. 



The method of preparing the gossypol and other feeds is shown in the 

 accompanying outline. 



OUTLINE OF METHOD OF PREPARING FEEDS 



Whole cottonseed. 

 Decorticated at mill. 



Hulls. 



Kernels, ground and sifted. 



"Fines" (100 per cent of 

 kernels). 



Extracted with petroleum 

 solvents. 



BrowTiish oil (34 per cent of kernels), 

 feed 290a. 



Residue I (66 per cent of 

 kernels), feed 290. 



Extracted with ethyl ether. 



Gossypol extract 



(2 per cent of kernels), 



feed 318. 



Residue II (64 per cent of 

 kernels), feed 316. 



Precipitated with 

 petroleum ether. 



Treated with 

 glacial acetic acid. 



Precipitated gossypol, Filtrate, 

 feed 320. feed 340. 



Recrystallized gossypol< 



"acetate," 



feed 321. 



-Crystalline 



gossypol 



"acetate." 



feed 319. 



Feeds 290, 318, 319, 320, and 321 are very toxic. 

 Feed 316 is very slightly toxic after long feeding. 

 Feeds 331 and 340 are nontoxic. 



Extracted with boiling 

 alcohol. 



Extract, Residue III, 



feed 330. feed 331. 



