Nov. IS, ig 



Gossypol, the Toxic Substance in Cottonseed Meal 269 



Rabbit 928. — Fatal in three hours. Excess discolored serous fluid in abdomen 

 containing oily globules. Moderate injection in intestines at points. Slight excess of 

 fluid. Lungs slightly congested and slightly edematous. 



Rabbit 929. — Died during night between the second and the thirteenth hoiu. 

 Excess brownish serum in abdominal cavity. Small intestines show areas of marked 

 injection. Lungs congested and somewhat edematous. 



Rabbit 930. — Slightly indisposed on following day and normal thereafter. Appetite 

 only slightly affected. 



Rabbit 933. — Same as 930. 



Feeding Gossypol Extract with Corn Meal and Molasses 



An artificial cottonseed meal was made by pouring the concentrated 

 ether extract of cottonseed kernels over corn meal. The daily feed for 

 each of four rabbits was estimated to be equivalent to 30 gm. of cotton- 

 seed kernels, and for each of two others, 15 gm. Control animals were 

 given corn meal and molasses. All the animals were supplied liberally 

 with green feed (pea vines, cabbage, and collards) in the morning. In 

 the afternoon (4 or 5 p. m.) they were given the various feeds mixed with 

 molasses. The controls on corn meal and molasses did well, gained in 

 weight, and need not be further mentioned. The gossypol extract 

 proved very toxic. The animals receiving the equivalent of 30 gm. of 

 cottonseed kernels refused to eat the cottonseed feed after the fifth day. 

 They began to refuse green feed later, became sicker, and the last one 

 died within 1 5 days. The two rabbits and a guinea pig receiving smaller 

 doses were soon made sick. One rabbit and a guinea pig refused the feed 

 thereafter, and the other rabbit died. See Table IV. 



Table IV. — Results of feeding gossypol extract {feed 318) with corn meal and molasses 

 to rabbits and guinea pig o 



DAILY FEED EQUIVALENT TO 30 GM. OF COTTONSEED KERNELS 



o I gm. of the mixture of feed 318 and dry corn meal is equivalent to approximately 2 gm of 

 kernels. 



