270 



Journal of Agricultural Research 



Table IV. — Result of feeding gossypol extract {feed 318) with corn meal and molasses 

 to rabbits and guinea pig — Continued 



DAILY FEED EQUIVALENT TO 15 GM. OP COTTONSEED KERNELS 



POST-MORTEM OBSERVATIONS 



Rabbit 941. — Reddish serum in abdominal cavity, 15 c. c. Cecum deeply injected. 

 Liver congested. Lungs slightly congested and edematous. Conspicuous thrombus 

 in right auricle. 



Rabbit 942. — Excess abdominal fluid, 15 c. c. Hemorrhagic (inflamed) and ulcer- 

 ated condition at pyloric end of small intestines. Large thrombus in right auricle. 



Rabbit 943. — Slight excess of abdominal fluid. Large intestines had some hemor- 

 rhagic areas. Liver congested. 



Rabbit 944. — Reddish serum in abdomen, 25 c. c. Serous membrane injected. 

 Small intestines reddened. Small thrombi present. Death due to enteritis. 



Rabbit 953. — Mesenteric blood vessels injected. Viscera practically normal. 

 Liver much congested. Kidneys much congested. 



Rabbit 954. — Experiment discontinued because animal refused to eat feed 318 

 after the eighth day. Subsequently put on precipitated gossypol. 



Guinea pig A. — Experiment discontinued because animal refused to eat feed 318. 



In order to ascertain the effect of a large dose, a large healthy rabbit 

 (945) was taken from the control feed and given all of feed 318 that it 

 would eat. It consumed 40 gm., equivalent to 80 gm. of kernels, on the 

 first day and was made sick on the following day. When it began to 

 recover on the fourth day it was given a small feed and died on the 

 ninth day, having lost considerably in weight. The protocol of rabbit 

 945 is as follows : 



September 23, p. m. — Ate 40 gm. of feed 318 with molasses. 

 September 24. — Appears sick; has diarrhea. Ate little green; refuses feed 318. 

 September 25. — Seems indisposed; refuses feed 318. 



September 26.— Better; eats cabbage. Weight 2,700 gm. Given 15 gm. of feed 318 

 and 15 gm. of com meal with molasses. Ate equivalent to 7 gm. of feed 318. 

 September 27, 28, and 29. — Eats pea vines readily. 



September 30. — Refuses green; p. m., ate com meal and molasses readily. 

 October i, a. m. — Refuses green. Died ninth day about 3 p. m. Weight, 2,410 gm. 

 Post-mortem examination showed considerable excess fluid in abdominal cavity. 



