272 



Journal of Agricultural Research 



Vol. V, No. 7 



A week after recovery it was started on feed 319 (precipitated gossypol 

 on com meal). We planned to have it eat 0.3 gm. of gossypol daily. The 

 first week 0.38 gm. of precipitated gossypol was eaten, the second week 

 0.67 gm., and only 0.60 gm. thereafter, a total of 1.65 gm. Death ensued 

 after 19 days. The animal ate feed 319 sparingly and very irregularly. 



A young rabbit (962) was fed similarly at the rate, of 0.14 gm. a day. 

 By weeks it ate, respectively, 0.97, 0.15, 0.15, and 0.34 gm. of precipitated 

 gossypol. It was normal after the first week and died on the twenty- 

 ninth day. 



A guinea pig refused to do anything more than nibble feed 318 (gossy- 

 pol extract), eating in 29 days only 34 gm. of the feed. It could not 

 be induced to eat feed 319 (precipitated gossypol) any better, consuming 

 only 1. 1 3 gm. in 27 days. The autopsy showed that a mesenteric twist 

 had cut off the blood supply of the last half or third of the intestines, 

 so that death was not directly traceable to the feed. 



Rabbit 949 was fed a large dose (1.44 gm.) of the precipitated gossypol 

 mixed with corn meal and molasses. The next two days it suffered 

 from diarrhea and refused to eat this feed, but it ate green feed. There- 

 after it was given precipitated gossypol in small doses, but it usually 

 refused all or part of this. Steadily losing weight, the animal died after 

 35 days, having eaten a total of 4.47 gm. of gossypol, inclusive of the 

 large dose. The amounts eaten each week were, respectively, 2.08, 

 0.58, 0.50, and 0.68 gm. 



Rabbit 937 had previously eaten the ether-extracted residue (feed 

 316) for 61 days and had increased in weight. Then, after several days 

 on corn meal and molasses the rabbit was fed precipitated gossypol. 

 We planned to feed 0.3 gm. a day, but only on three days did it eat this 

 amount, usually refusing it entirely or in part. After 2 1 days a crys- 

 talline product was substituted for precipitated gossypol. The animal 

 steadily decreased in weight and died after 33 days. The total amount 

 of gossypol consumed was 2.52 gm. By weeks, 1.19, 0.27, 0.5, 0.57, 

 and o gm. of gossypol were consumed. It ate practically nothing 

 during the last 8 days. See Table V. 



Table V. — Results of feeding precipitated gossypol with corn meal and molasses {feed jiq) 

 to rabbits and guinea pigs 



Rabbit 954 . . 

 Rabbit 961 . . 

 Rabbit 962 . . 

 Guinea pig A 

 Rabbit 949 . . 

 Rabbit 937 . . 



Died. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 



oThis quantity (4.47 gm.) includes a large dose of 1.44 gm. which evidently passed the bowel quickly. 



