274 



Journal of Agricultural Research 



Vol. V, No. 7 



To prepare the injection, 0.7 gm. of this substance was dissolved in 

 ether and the ethereal solution mixed with 20 c. c. of purified cottonseed 

 oil. The clear reddish yellow solution was wanned over steam until it 

 had not the slightest odor of ether. This was then injected in doses of 

 10 c. c. into two rabbits, 963 and 964, weighing 1,560 and 1,485 gm., 

 respectively. In a few minutes the rabbits became very uneasy and 

 then passed into a sort of stupor. Rabbit 963 died in 3.5 hours and 964 

 in 4.5 hours. The death of rabbit 964 was witnessed. Shortly before 

 death it toppled over on its side, had several convulsions, gasped several 

 times, squealed, and died. 



In these cases, as in the previous one, there was considerable injecta 

 left in the abdominal cavity. See Table VI. 



Table VI. — Result of administering crystalline gossypol "acetate " intraperitoneally in 

 cottonseed oil to rabbits 



CRYSTALtlNE GOSSYPOL "ACETATE" 



Initial 

 weight of 

 rabbit. 



Weight of 

 gossyix)!. 



Dose 

 volume. 



Weight of 

 gossypol 

 per kilo 

 of body 



weight. 



955- 

 956. 



Gm. 



1,115 



1,180 



Gm. 



0.493 



Died. 

 Do. 



RECRYSTALLIZED GOSSYPOL "ACETATE" 



963' 

 964. 



I, 560 

 1,485 



0-35 

 •35 



0.244 



•235 



Died. 

 Do. 



POST-MORTEM OBSERVATIONS 



Rabbits 955 and 956. — Dead and cold after six hours. Apparent nonabsorption of 

 much of the injection. Excess of fluid. Peritoneum stained brown. Visceral blood 

 vessels slightly injected. 



Rabbit 963. — Died in convulsions. Part of injecta present as oily globules. Serum 

 present also. Serous membrane stained yellow. 



Rabbit 964. — Same as 963, except small intestines were rather markedly injected. 



Feeding Crystalline Gossypol "Acetate" to Rabbits 

 Crystalline gossypol "acetate" with corn meal and molasses (feed 

 319) was fed to rabbit 965. The feed was refused on the fourth day, after 

 which it was not further given. Only on the first day did the animal 

 eat the entire amount fed. After eating 0.3 gm. of crystalHzed gossypol 

 "acetate," it had a bad diarrhea and little appetite for green feed the next 

 day. The protocol was as follows : 



December 15, first day. — ^Ate 0.3 gm. with com meal and molasses; weight, 2,340 



gm. 



December 16, second day. — Bad diarrhea, and cats little green feed. 



