276 



Journal of Agricultural Research 



Vol. V, No. 7 



tion. Gossypol from old seeds is greenish. The post-mortem examina- 

 tion showed considerable irritation in the small intestines of these 

 rabbits. 



EFFECT OP SMALL DOSES OF CRYSTALLINE GOSSYPOL "ACETATE" 



Rabbit 978, weight 2,100 gm., was fed on recrystallized gossypol 

 " acetate " at the rate of 0.05 gm. daily mixed with com meal and molasses. 

 After one week it began to show a diminished appetite for the feed. On 

 the nineteenth day it was given a double dose by mistake, and for two 

 weeks thereafter showed a very poor appetite for the feed. At that time 

 it weighed i ,820 gm. 



Its record by weeks is as follows : 



Quantity of gossypol eaten gm. . 0.34; 0.33; 0.335; 0.25; 0.25; 0.32; 



0.125. 

 Weight of rabbit gm.. 2,045; 2,010; 2,070; 1,820; 1,930; 



1.730- 

 Total quantity of gossypol eaten gm. . 1.95. 



The animal died after 5 1 days. The post-mortem examination showed 

 that a convolvulus had set up a necrotic condition in the intestine. 

 Whether the feed was contributory to this condition we are unable to 

 say. See Table VII. 



Table VII. — Results of feeding crystalline gossypol "acetate " (feed jig) to rabbits 



Feed and rabbit No. 



Gossypol ' ' acetate ' ' : 



965 



951 



965A 



Recrystallized gossj'pol 

 "acetate": 



974 



976 



977 



972 



Gossypol ' ' acetate ' ' fed 

 in small doses (0.05 

 gm. per day): 



978 



Made sick. 

 Died. 

 Do. 



Made sick. 

 Died. 



Do. 



Do. 



Died from second- 

 ary^ causes. 



Feeding Gossypol "Acetate" to Fowls ' 



Two cockerels (986 and 987) previously fed on cottonseed meal to 

 study the symptoms, were started on gossypol. Powdered gossypol 

 in 0.3 gm. doses was fed, followed by a little water. On the fourth 



1 This experiment was carried on under the supervision of Dr. B. P. Kaupp, Pathologist, of the Poul- 

 try Division, North Carolina Agricultural Experiment Station. 



