Angular Leaf -Spot of Cucumbers 



467 



Table I. --Differences between Bacterium lackrj'^nans and Burger's cucumber organism 



GEOGRAPHICAL DISTRIBUTION OF THE DISEASE 



Mr. Frederick V. Rand, of this laboratory, by whom these specimens 

 were collected, reported the disease in 1915, from the following localities: 



Michigan: Big Rapids, Muskegon, Grand Haven, Holland, Grand Rapids, and 

 Hudson ville. 



Indiana: Plymouth, Monterey, Tyner, and Donaldson. 

 Wisconsin: Racine, Portage, Ripon, Princeton, and Milwaukee. 

 New York: Constable, Malone, North Lawrence, and Long Island. 

 Canada: Provinces of Ontario and Quebec. 



In regard to the amount of injury caused by this disease, Mr. Rand says : 



In most cases I found the angular leaf-spot causing a rather minor injury, but in an 

 occasional field I fotind all the leaves back of the tips of the vines very badly shot- 

 holed and presenting an exceedingly ragged appearance, such that serious injury 

 to the crop must inevitaby result. Last year this disease had done more damage 

 than any other in the vicinity of Ripon, Wis. 



This disease has also been reported recently from Maryland and several 

 other Southern States. 



Earlier the senior writer received specimens from Michigan, Wisconsin^ 

 Indiana, Connecticut, and the District of Columbia. 



INOCULATION EXPERIMENTS 



On October 26, 1914, young cucumber plants were sprayed in cages in 

 the hothouse with water suspensions from young agar slants made from 

 three colonies on the plates poured from diseased leaves. The plants 

 were kept moist in the cages for 30 hours, then removed to the bench. 



