530 



Journal of Agricultural Research 



In order to understand more fully the different tabulations, a brief 

 description of each sample follows. 



Nos. 285, 812, and 852 were collected in April of 1912, 1913, and 1914, 

 respectively, from the cut end of the same main branch about 20 feet 

 from the root of the vine and just after the sap flow commenced. 



No. 853 was collected in April, 1914, from the cut end of another main 

 branch about 4 feet from the root of the vine and just after the sap flow 

 commenced. This sample was taken at the same time as No. 852. 



No. 854 was collected in April, 1914, from the same point as No. 852, 

 but seven days later and just before the sap flow ceased. 



No. 900 was collected in April, 1915, from the cut end of one of the main 

 branches about 20 feet from the root of the vine and just after the sap 

 flow commenced. This was a diff'erent branch from that from which No. 

 285 was taken, because no sap exuded from the old branch, and it seemed 

 to have been greatly weakened by the annual loss. 



No. 901 was collected in April, 1915, from several of the small branches 

 or shoots which were of the previous year's growth and just after the sap 

 flow commenced. This sample was taken at the same time as No. 900 

 and from 10 shoots which were located several feet from the main branches. 



Nos. 902, 904, and 906 were collected for three successive days from 

 9 a. m. to 5 p. ra., beginning on April 29, 1915, four days after and from 

 the same point as No. 900. 



Nos. 903, 905, and 907 were collected for three successive nights from 

 5 p. m. to 9 a. m., beginning on April 29, 1915, and from the same point 

 as No. 900. 



The variation in the percentage composition of the fresh sap arid the 

 ash of samples 852, 853, 900, and 901 are given in Tables I and II. 



Table I.- 



■Variation in percentage composition of fresh sap collected at the same time 

 from, different points on the wild-grape vine ' 



' Nos. S52 and 853 were collected in 1914; Nos. 900 and 901 in 1915. 



