48 Journal of Agricultural Research voi. xvni, No. i 



portant feature of the new method is the use of ice water for the extrac- 

 tion of the material. 



(3) By means of the ice- water method it is shown that the amount 

 of acid present in oat kernels does not change markedly during the 

 early stages of spoilage. If oats are sulphured their acidity is increased. 



(4) Oats which previously had been sulphur bleached showed no 

 increase in acidity upon prolonged standing in the ground state when 

 tested by means of the ice-water method, the acid-forming ferments of 

 the grain having been destroyed by the sulphur fumes. With Schindler's 

 method pronounced increases in the titration values were still observed 

 in these cases, owing doubtless to the fact that certain protein cleavage 

 products continue to be formed, which in aqueous solution are ampho- 

 theric, but which possess an acid reaction in the presence of alcohol. 



(5) Ice-water extracts of oats or com, if filtered and kept at the 

 temperature of 1° to 2° C. for 24 hours without being neutralized, undergo 

 no change in acidity. If neutralized, a new formation of acid takes 

 place, notwithstanding the low temperature. 



LITERATURE CITED 



(i) Adler, Ludwig. 



1914. ZUR BESTIMMUNG DES AMrNOSAURE- UND POLYPEPTm-STICKSTOFFS IN 



GERSTE, MALZ UND BIER MITTELS DER PORMOLTITRATION. In Ztschr. 



Gesam. Brauw., n. F. Jahrg. 37, No. 9, p. 105-108, fig. 15; No. 10, 

 p. 117-121; No. II, p. 129-133. 



(2) 



191 5. tJBER DIE PHOSPHATASEN IM MALZ. In Biochem. ztschr., Bd. 70, Heft 



1/2, p- 1-36. 



(3) 



1915. tJBER DIE POLYPEPTID- UND AMINOSAURE-LIEFERNDEN ENZYME IM MALZ. 



In Ztschr. Gesam. Brauw., n. F. Jahrg. 38, No. 17, p. 129-131; No. 

 18, p. 137-142; No. 19, p. 146-149; No. 20, p. 153-155- 

 (4) 



1915. VERWENDUNG EINES KOLORIMETERS ZUR FORMOLTITRATION. In Ztschr. 



Gesam. Brauw., n. F. Jahrg. 38, No. 31, p. 241-243; No. 32, p. 251-252. 

 (5) Besley, H. J., and Baston, G. H. 



1914. ACIDITY as a FACTOR IN DETERMINING THE DEGREE OP SOUNDNESS OF 



CORN. U. S. Dept. Agr. Bui. 102, 45 p., 33 fig. 

 (6) 



1916. IMPROVED APPARATUS FOR USE IN MAKING ACIDITY DETERMINATIONS OF 



CORN. U. S. Dept. Agr. Off. Sec. Circ. 68, 4 P-, i fig- 



(7) BiRCKNER, Victor. 



1919. ACIDIMETRIC TITRATION OF GRAIN EXTRACTS AND ANIMO ACIDS IN THE 

 PRESENCE OF ALCOHOL, 

 /n Jour. Biol. Chem., v. 38, no. 2, p. 245-254, 



(8) BLACK, O. F., and Alsberg, C. L. 



I9IO. THE DETERMINATION OF THE DETERIORATION OF MAIZE WITH INCIDENTAL 



REFERENCE TO PELLAGRA. U. S. Dept. Agr. Bur. Plant Indus. Bui. 

 199, 36 p. 



